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dc.creatorAngulo Ugalde, Yamileth
dc.creatorChaves Olarte, Esteban
dc.creatorRucavado Romero, Alexandra
dc.creatorGutiérrez, José María
dc.creatorLomonte, Bruno
dc.creatorAlape Girón, Alberto
dc.date.accessioned2017-01-16T19:36:10Z
dc.date.available2017-01-16T19:36:10Z
dc.date.issued1997-03
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0003986196998653es_ES
dc.identifier.issn0003-9861
dc.identifier.urihttp://hdl.handle.net/10669/29425
dc.description.abstractA new myotoxic phospholipase A2was isolated from the venom of the arboreal snakeBothriechis schlegelii(formerlyBothrops schlegelii) from Costa Rica, by ion-exchange chromatography on CM-Sephadex.B. schlegeliimyotoxin I is a basic protein (pI> 9.3) with a subunit molecular weight of 15 kDa, which migrates as a dimer in sodium dodecyl sulfate–polyacrylamide gel electrophoresis under nonreducing conditions. This myotoxin is recognized by antibodies generated againstBothrops aspermyotoxin II (a lysine-49 phospholipase A2), by both enzyme-immunoassay and gel immunodiffusion, in the latter case with a pattern of partial identity. The toxin induces rapid myonecrosis upon intramuscular injection in mice, as evidenced by the early increase in plasma creatine kinase activity and by direct intravital microscopic observation.B. schlegeliimyotoxin I also induces edema in the mouse footpad assay and exerts lethal activity (LD50∼2.5 μg/g) upon intravenous injection. The toxin has a low phospholipase A2activity (4.2 μEq·mg−1·min−1) using egg yolk phospholipids as substrate. It also shows a weak anticoagulant effectin vitro.Its N-terminal sequence, SMYELGKMILLETGKNAATSYIAYG, shows 93% homology with bothBothrops aspermyotoxin II andB. jararacussubothropstoxin I, suggesting thatB. schlegeliimyotoxin I may be a new lysine-49 variant of this family of myotoxic phospholipases A2.es_ES
dc.description.sponsorshipUniversidad de Costa Rica Proy. 741-95-264 y 741-96-265 International Foundation for Science F/1388-3Fes_ES
dc.language.isoen_USes_ES
dc.sourceArchives of Biochemistry and Biophysics; Volumen 339, Número 2, 1997es_ES
dc.subjectMyotoxines_ES
dc.subjectPhospholipases A2es_ES
dc.subjectBothriechises_ES
dc.subjectBothropses_ES
dc.subjectSnake venomes_ES
dc.titleIsolation and characterization of a myotoxic phospholipase A2 from the venom of the arboreal snake Bothriechis (Bothrops) schlegelii from Costa Ricaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1006/abbi.1996.9865
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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