Listar Microbiología por autor "9b8320ba-4241-4f7e-9982-b24409e85c45"
Mostrando ítems 1-17 de 17
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Amino acid sequence and crystal structure of BaP1, a metalloproteinase from Bothrops asper snake venom that exerts multiple tissue-damaging activities
Watanabe, Leandra; Shannon, John D.; Valente, Richard H.; Rucavado Romero, Alexandra; Alape Girón, Alberto; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Gutiérrez, José María; Arni, Raghuvir K. (2003-10)BaP1 is a 22.7-kD P-I-type zinc-dependent metalloproteinase isolated from the venom of the snake Bothrops asper, a medically relevant species in Central America. This enzyme exerts multiple tissue-damaging activities, ... -
Biochemical characterization and pharmacological properties of a phospholipase A2 myotoxin inhibitor from the plasma of the snake Bothrops asper
Lizano González, Sergio; Lomonte, Bruno; Fox, Jay W.; Gutiérrez, José María (1997-09-15)A protein that neutralizes the biological activities of basic phospholipase A2 (PLA2) myotoxin isoforms from the venom of the snake Bothrops asper was isolated from its blood by affinity chromatography with Sepharose-immobilized ... -
Characterization of 'basparin A,' a prothrombin-activating metalloproteinase, from the venom of the snake Bothrops asper that inhibits platelet aggregation and induces defibrination and thrombosis
Loría Masís, Gilbert David; Rucavado Romero, Alexandra; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Alape Girón, Alberto; Gutiérrez, José María (2003-10)A prothrombin activator, named ‘basparin A,’ was isolated from the venom of the crotaline snake Bothrops asper, the species responsible for the majority of snakebite cases in Central America. It is an acidic (pI 5.4), 70 ... -
Characterization of aspercetin, a platelet aggregating component from the venom of the snake Bothrops asper which induces thrombocytopenia and potentiates metalloproteinase-induced hemorrhage
Rucavado Romero, Alexandra; Soto Morera, Mónica; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Escalante Muñoz, Teresa; Gutiérrez, José María (2001-04)Thrombocytopenia occurs in a number of patients bitten by Bothrops asper, a species responsible for the majority of snakebites in Central America and southern Mexico. In this work we describe the isolation of a new ... -
Efficacy of IgG and F(ab′)2 Antivenoms to Neutralize Snake Venom-induced Local Tissue Damage as Assessed by the Proteomic Analysis of Wound Exudate
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Shannon, John D.; Ayala Castro, Carla N.; Villalta Arrieta, Mauren; Gutiérrez, José María; Fox, Jay W. (2012)Proteomic analysis of wound exudates represents a valuable tool to investigate tissue pathology and to assess the therapeutic success of various interventions. In this study, the ability of horse-derived IgG and F(ab0)2 ... -
High resolution analysis of snake venom metalloproteinase (SVMP) peptide bond cleavage specificity using proteome based peptide libraries and mass spectrometry
Paes Leme, Adriana F.; Escalante Muñoz, Teresa; Pereira, José G. C.; Oliveira, Ana K.; Sanchez, Eladio F.; Gutiérrez, José María; Serrano, Solange M. T.; Fox, Jay W. (2011-04-01)Both serine and metalloproteinases have been shown to play the role of toxins in the venoms of many snakes. Determination of the natural protein substrates of these toxins is an important feature in the toxinological ... -
Key events in microvascular damage induced by snake venom hemorrhagic metalloproteinases
Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Fox, Jay W.; Gutiérrez, José María (2011-08-24)Hemorrhage is one of the most significant effects in envenomings induced by viperid snakebites. Damage to the microvasculature, induced by snake venom metalloproteinases (SVMPs), is the main event responsible for this ... -
Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis
Herrera Arias, Cristina; Macêdo, Jéssica Kele A.; Feoli Grant, Andrés; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Gutiérrez, José María; Fox, Jay W. (2016-04-01)The time-course of the pathological effects induced by the venom of the snake Bothrops asper in muscle tissue was investigated by a combination of histology, proteomic analysis of exudates collected in the vicinity of ... -
Novel insights into capillary vessel basement membrane damage by snake venom hemorrhagic metalloproteinases: A biochemical and immunohistochemical study
Escalante Muñoz, Teresa; Shannon, John D.; Moura Da Silva, Ana M.; Gutiérrez, José María; Fox, Jay W. (2016-11-15)The hemorrhagic activity characteristic of viperid snake envenomations is due to the action of venom metalloproteinases (SVMPs) on the capillary vessel basement membrane (BM). This study compared the action of two SVMPs ... -
Proteomics of Wound Exudate in Snake Venom-Induced Pathology: Search for Biomarkers To Assess Tissue Damage and Therapeutic Success
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Shannon, John D.; Gutiérrez, José María; Fox, Jay W. (2011-02-10)Tissue damage analysis by traditional laboratory techniques is problematic. Proteomic analysis of exudates collected from affected tissue constitutes a powerful approach to assess tissue alterations, since biomarkers ... -
Role of collagens and perlecan in microvascular stability: exploring the mechanism of capillary vessel damage by snake venom metalloproteinases
Escalante Muñoz, Teresa; Ortiz Chaves, Natalia; Rucavado Romero, Alexandra; Sánchez, Eladio; Richardson, Michael; Fox, Jay W.; Gutiérrez, José María (2011-12-08)Hemorrhage is a clinically important manifestation of viperid snakebite envenomings, and is induced by snake venom metalloproteinases (SVMPs). Hemorrhagic and non-hemorrhagic SVMPs hydrolyze some basement membrane (BM) and ... -
Role of the snake venom toxin jararhagin in proinflammatory pathogenesis: In vitro and in vivo gene expression analysis of the effects of the toxin
Gallagher, Paul; Bao, Yongde; Serrano, Solange M. T.; Laing, Gavin D.; Theakston, R. David G.; Gutiérrez, José María; Escalante Muñoz, Teresa; Zigrino, Paola; Moura Da Silva, Ana M.; Nischt, Roswitha; Mauch, Cornelia; Moskaluk, Christopher; Fox, Jay W. (2005-09)To assess the indirect effects of snake venom metalloproteinases (SVMP) on host tissue local necrosis, we investigated the effect of the SVMP jararhagin on the gene expression profiles of human fibroblasts in vitro and ... -
Tissue Localization and Extracellular Matrix Degradation by PI, PII and PIII Snake Venom Metalloproteinases: Clues on the Mechanisms of Venom-Induced Hemorrhage
Voisin, Mathieu-Benoit; Rucavado Romero, Alexandra; Morazán, Diego; Macêdo, Jéssica Kele A.; Calvete Chornet, Juan José; Sanz, Libia; Nourshargh, Sussan; Gutiérrez, José María; Fox, Jay W.; Herrera Arias, Cristina; Escalante Muñoz, Teresa (2015-04-24)Snake venom hemorrhagic metalloproteinases (SVMPs) of the PI, PII and PIII classes were compared in terms of tissue localization and their ability to hydrolyze basement membrane components in vivo, as well as by a ... -
Tissue pathology induced by snake venoms: How to understand a complex pattern of alterations from a systems biology perspective?
Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Lomonte, Bruno; Angulo Ugalde, Yamileth; Fox, Jay W. (2010-01)The study of snake venom-induced local tissue pathology has been predominantly based on experimental designs and concepts that view tissue alterations from a dichotomous, homogeneous and reductionist perspective. Experimental ... -
Two phospholipase A2 inhibitors from the plasma of Cerrophidion (Bothrops) godmani which selectively inhibit two different group-II phospholipase A2 myotoxins from its own venom: isolation, molecular cloning and biological properties
Lizano González, Sergio; Angulo Ugalde, Yamileth; Lomonte, Bruno; Fox, Jay W.; Lambeau, Gérard; Lazdunski, Michel; Gutiérrez, José María (2000-03-15)Myotoxic phospholipases A2 (PLA2s; group II) account for most of the muscle-tissue damage that results from envenomation by viperid snakes. In the venom of the Godman's viper (Cerrophidion godmani, formerly Bothrops godmani), ... -
Unresolved issues in the understanding of the pathogenesis of local tissue damage induced by snake venoms
Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Herrera Arias, Cristina; Fernández Ulate, Julián; Lomonte, Bruno; Fox, Jay W. (2018-06)Snakebite envenoming by viperid species, and by some elapids, is characterized by a complex pattern of tissue damage at the anatomical site of venom injection. In severe cases, tissue destruction may be so extensive as ... -
Wound exudate as a proteomic window to reveal different mechanisms of tissue damage by snake venom toxins
Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Pinto, Antonio F. M.; Terra, Renata M. S.; Gutiérrez, José María; Fox, Jay W. (2009)In light of the complexity of wound tissue, proteomic analysis may not clearly reveal the nature of the wound or the processes involved in healing. However, exudate associated with wounds may provide a “window” on cellular ...