Listar Microbiología por autor "81f81be3-31dc-4d32-846d-fcf817ada6b2"
Mostrando ítems 1-8 de 8
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Cross-recognition of a pit viper (Crotalinae) polyspecific antivenom explored through high-density peptide microarray epitope mapping
Engmark, Mikael Gerling; Lomonte, Bruno; Gutiérrez, José María; Laustsen, Andreas Hougaard; de Masi, Federico; Andersen, Mikael Rørdam; Lund, Ole (2017-07)Snakebite antivenom is a 120 years old invention based on polyclonal mixtures of antibodies purified from the blood of hyper-immunized animals. Knowledge on antibody recognition sites (epitopes) on snake venom proteins ... -
Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis
Laustsen, Andreas Hougaard; Gutiérrez, José María; Rasmussen, Arne R.; Engmark, Mikael Gerling; Gravlund, Peter; Sanders, Kate L.; Lohse, Brian; Lomonte, Bruno (2015-12-01)Four specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF ... -
Exploration of immunoglobulin transcriptomes from mice immunized with three-finger toxins and phospholipases A2 from the Central American coral snake, Micrurus nigrocinctus
Laustsen, Andreas Hougaard; Engmark, Mikael Gerling; Clouser, Christopher; Timberlake, Sonia; Vigneault, Francois; Gutiérrez, José María; Lomonte, Bruno (2017)Snakebite envenomings represent a neglected public health issue in many parts of the rural tropical world. Animal-derived antivenoms have existed for more than a hundred years and are effective in neutralizing snake venom ... -
From Fangs to Pharmacology: The Future of Snakebite Envenoming Therapy
Laustsen, Andreas Hougaard; Engmark, Mikael Gerling; Milbo, Christina; Johannesen, Jonás; Lomonte, Bruno; Gutiérrez, José María; Lohse, Brian (2016)The snake is the symbol of medicine due to its association with Asclepius, the Greek God of medicine, and so with good reasons. More than 725 species of venomous snakes have toxins specifically evolved to exert potent ... -
High-density peptide microarray exploration of the antibody response in a rabbit immunized with a neurotoxic venom fraction
Engmark, Mikael Gerling; Jespersen, Martin Closter; Lomonte, Bruno; Lund, Ole; Laustsen, Andreas Hougaard (2017-11)Polyvalent snakebite antivenoms derive their therapeutic success from the ability of their antibodies to neutralize venom toxins across multiple snake species. This ability results from a production process involving ... -
High-throughput immuno-profiling of mamba (Dendroaspis) venom toxin epitopes using high-density peptide microarrays
Engmark, Mikael Gerling; Andersen, Mikael Rørdam; Laustsen, Andreas Hougaard; Patel, Jigar; Sullivan, Eric; de Masi, Federico; Hansen, Christian S.; Kringelum, Jens V.; Lomonte, Bruno; Gutiérrez, José María; Lund, Ole (2016-11-08)Snakebite envenoming is a serious condition requiring medical attention and administration of antivenom. Current antivenoms are antibody preparations obtained from the plasma of animals immunised with whole venom(s) and ... -
Pros and cons of different therapeutic antibody formats for recombinant antivenom development
Laustsen, Andreas Hougaard; Gutiérrez, José María; Knudsen, Cecilie; Johansen, Kristoffer H.; Bermúdez Méndez, Erick; Cerni, Felipe A.; Jürgensen, Jonas A.; Ledsgaard, Line; Martos Esteban, Andrea; Øhlenschlæger, Mia; Pus, Urska; Andersen, Mikael Rørdam; Lomonte, Bruno; Engmark, Mikael Gerling; Pucca, Manuela Berto (2018-05)Antibody technologies are being increasingly applied in the field of toxinology. Fuelled by the many advances in immunology, synthetic biology, and antibody research, different approaches and antibody formats are being ... -
Selecting key toxins for focused development of elapid snake antivenoms and inhibitors guided by a Toxicity Score
Laustsen, Andreas Hougaard; Lohse, Brian; Lomonte, Bruno; Engmark, Mikael Gerling; Gutiérrez, José María (2015-09-15)For more than 100 years, antivenoms have been produced by traditional methods of immunization of large mammals with mixtures of snake venoms (World Health Organization, 2010 and Gutiérrez et al., 2011). With the introduction ...