Listar Microbiología por autor "627e1820-b0a1-4e72-b340-07d223d27ee0"
Mostrando ítems 1-20 de 68
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A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms
Otero Patiño, Rafael; Gutiérrez, José María; Rojas Céspedes, Gustavo; Núñez Rangel, Vitelbina; Díaz, Abel; Miranda, E.; Uribe, A. F.; Silva, Juan F.; Ospina, J. G.; Medina, Y.; Toro, María Fabiola; García, Martha E.; León Montero, Guillermo; García, M.; Lizano González, Sergio; De La Torre, J.; Márquez, J.; Mena, Y.; González, N.; Arenas, L. C.; Puzón, A.; Blanco, N.; Sierra, A.; Espinal, M. E.; Arboleda, Margarita; Jiménez, J. C.; Ramírez, P.; Díaz, M.; Guzmán, M. C.; Barros, J.; Henao, S.; Ramírez, A.; Macea, U.; Lozano, R. (1999-06)A randomized blinded clinical trial was performed in 53 patients bitten by Bothrops sp. and Porthidium sp. in Antioquia and Chocó, Colombia, in order to compare the efficacy and safety of two antivenoms made of whole IgG ... -
Anti-human erythrocyte antibodies in horse-derived antivenoms used in the treatment of snakebite envenomations
León Montero, Guillermo; Rodríguez, Miguel Angel; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María (2007-03)This work examined the presence of antibodies reacting with human erythrocytes in horse-derived antivenoms used in the treatment of snakebite envenomations, and assessed the efficacy of various fractionation protocols in ... -
Anticomplementary activity of equine whole IgG antivenoms: comparison of three fractionation protocols
León Montero, Guillermo; Lomonte, Bruno; Gutiérrez, José María (2005-01)Early adverse reactions occur in a number of patients treated with heterologous antivenoms and have been associated with anticomplementary activity (ACA). In order to reduce the ACA of equine whole IgG antivenoms produced ... -
Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa
Calvete Chornet, Juan José; Cid, Pedro; Sanz, Libia; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Herrera Vega, María; León Montero, Guillermo; Harrison, Robert A.; Durfa, Nandul; Nasidi, Abdulsalami; Theakston, R. David G.; Warrell, David A.; Gutiérrez, José María (2010)The immunoreactivity of EchiTAb-Plus-ICP, an antivenom developed for the treatment of snakebite envenoming in sub-Saharan Africa, to venoms of seven Echis and Bitis species, was assessed by “antivenomics.” This proteomic ... -
Antivenomic Characterization of Two Antivenoms Against the Venom of the Taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia
Herrera Vega, María; Paiva, Owen K.; Pagotto, Ana Helena; Segura Ruiz, Álvaro; Serrano, Solange M. T.; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Jensen, Simon D.; León Montero, Guillermo; Williams, David J.; Gutiérrez, José María (2014-11-05)Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were ... -
Antivenoms for Snakebite Envenomings
Gutiérrez, José María; León Montero, Guillermo; Lomonte, Bruno; Angulo Ugalde, Yamileth (2011-10)Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. Antivenoms are manufactured by immunizing animals, usually horses, with venoms from a single or several medically-relevant snake ... -
Antivenoms for the treatment of snakebite envenomings: The road ahead
Gutiérrez, José María; León Montero, Guillermo; Burnouf, Thierry (2011-05)The parenteral administration of antivenoms is the cornerstone of snakebite envenoming therapy. Efforts are made to ensure that antivenoms of adequate efficacy and safety are available world-wide. We address the main issues ... -
Assessing a 6-h endpoint observation time in the lethality neutralization assay used to evaluate the preclinical efficacy of snake antivenoms
Durán Blanco, Gina; Solano Blanco, María Gabriela; Gómez Argüello, Aarón; Cordero Vásquez, Daniel; Sánchez Sánchez, Adriana; Villalta Arrieta, Mauren; Sánchez Chaves, Melvin; Díaz Oreiro, Cecilia; Gutiérrez, José María; León Montero, Guillermo (2021-11)The lethality neutralization assay in mice is the gold standard for the evaluation of the preclinical efficacy and specification fulfillment of snake antivenoms. However, owing to the animal suffering involved, this assay ... -
Assessing endotoxins in equine-derived snake antivenoms: Comparison of the USP pyrogen test and the Limulus Amoebocyte Lysate assay (LAL)
Solano Trejos, María Gabriela; León Montero, Guillermo; Gómez Argüello, Aarón (2015-08-29)Snake antivenoms are parenterally administered; therefore, endotoxin content must be strictly controlled. Following international indications to calculate endotoxin limits, it was determined that antivenom doses between ... -
Assessing the preclinical efficacy of antivenoms: From the lethality neutralization assay to antivenomics
Gutiérrez, José María; Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Sanz, Libia; Lomonte, Bruno; Calvete Chornet, Juan José; León Montero, Guillermo (2013-07)The assessment of the capacity of antivenoms to neutralize the lethal activity of snake venoms is the gold standard in the preclinical analysis of antivenom efficacy, and is routinely performed by manufacturers and quality ... -
Caracterización de la toxicidad de venenos de serpiente sobre nauplios de Artemia salina y su utilidad para la evaluación de la potencia neutralizante de antivenenos ofídicos.
Araya Pizarro, Xavier Alberto (2023-06-30)Los antivenenos ofídicos son soluciones de inmunoglobulinas purificadas a partir del plasma de animales que han sido inmunizados con veneno de serpiente y administrados oportunamente, constituyen la única herramienta ... -
Caracterización proteómica y toxicológica del veneno de Crotalus pifanorum (viperidae) y evaluación de su uso como inmunógeno sobre la respuesta por anticuerpos contra co-inmunógenos y venenos de otros crótalos americanos
Alfaro Chinchilla, Adriana (2019)En Costa Rica se produce un antiveneno poliespecífico (PoliVal-ICP) usando como inmunógenos los venenos de las serpientes Bothrops asper, Crotalus simus y Lachesis stenophrys. Este antiveneno es capaz de neutralizar a la ... -
Comparación entre inmunización pasiva e inmunización activa como procedimiento para controlar los envenenamientos producidos por la serpiente Bothrops asper (terciopelo) en un modelo canino
Chavarría Chan, Daniel (2020)Los accidentes ofídicos en animales domésticos son un problema importante para la garantía del bienestar animal en Costa Rica. En este trabajo se comparó la inmunización pasiva por administración de antivenenos con la ... -
Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity
Herrera Vega, María; Fernández Ulate, Julián; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; León Montero, Guillermo; Angulo Ugalde, Yamileth; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2012-04-03)The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of ... -
Comparative study of the efficacy and safety of two polyvalent, caprylic acid fractionated [IgG and F(ab0)2] antivenoms, in Bothrops asper bites in Colombia
Otero Patiño, Rafael; Segura Ruiz, Álvaro; Pereañez, Jaime Andrés; Herrera Vega, María; Angulo Ugalde, Yamileth; León Montero, Guillermo; Gutiérrez, José María; Barona, Jacqueline; Estrada, Sebastián; Quintana, Juan Carlos; Vargas Muñoz, Leidy Johana; Gómez, Juan Pablo; Díaz, Abel; Suárez, Ana María; Fernández, Jorge; Ramírez, Patricia; Fabra, Patricia E.; Perea, Monica; Fernández, Diego; Arroyo, Yobana; Betancur, Dalila; Pupo, Lady; Córdoba, Elkin; Ramírez, Eugenio; Arrieta, Ana Berta; Rivero, Alcides; Mosquera, Diana Carolina; Conrado, Nectty Lorena; Ortiz, Rosina (2012-02)The efficacy and safety of two polyvalent horse-derived antivenoms in Bothrops asper envenomings were tested in a randomized, double-blind, clinical trial performed in Colombia. Both antivenoms were manufactured from the ... -
Comparative study on the ability of IgG and F(ab')2 antivenoms to neutralize lethal and myotoxic effects induced by Micrurus nigrocinctus (coral snake) venom
León Montero, Guillermo; Stiles, Bradley G.; Alape Girón, Alberto; Rojas Céspedes, Gustavo; Gutiérrez, José María (1999-08)A comparative study was performed on the ability of IgG and F(ab')2 antivenoms to neutralize lethal and myotoxic activities of Micrurus nigrocinctus venom. Both antivenoms were adjusted to a similar neutralizing potency ... -
Comparative study on the ability of IgG and Fab sheep antivenoms to neutralize local hemorrhage, edema and myonecrosis induced by Bothrops asper (terciopelo) snake venom
León Montero, Guillermo; Valverde Barrantes, Juan Manuel; Rojas Céspedes, Gustavo; Lomonte, Bruno; Gutiérrez, José María (2000-02)The ability of sheep antivenoms, consisting of whole IgG molecules or Fab fragments, to neutralize local hemorrhage, edema and myonecrosis induced by Bothrops asper venom was comparatively studied in mice. The two antivenoms ... -
Comparison between IgG and F(ab′)2 polyvalent antivenoms: neutralization of systemic effects induced by Bothrops asper venom in mice, extravasation to muscle tissue, and potential for induction of adverse reactions
León Montero, Guillermo; Monge Monge, María; Rojas Umaña, Ermila; Lomonte, Bruno; Gutiérrez, José María (2001-06)Whole IgG and F(ab′)2 equine-derived polyvalent (Crotalinae) antivenoms, prepared from the same batch of hyperimmune plasma, were compared in terms of neutralization of the lethal and defibrinating activities induced by ... -
Comparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venom
Olmedo, Hidekel; Herrera Vega, María; Rojas Sánchez, Leonardo; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Leiguez, Elbio; Teixeira, Catarina de Fátima; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo; Montero Villalobos, Mavis Lili (2014)The adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro, most of the venom proteins are similarly ... -
Comparison of the adjuvant activity of emulsions with different physicochemical properties on the antibody response towards the venom of West African carpet viper (Echis ocellatus)
Valverde Barrantes, Juan Manuel; Rodríguez Mora, Karina María; Villalta Arrieta, Mauren; Herrera Vega, María; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Montero Villalobos, Mavis Lili; Gutiérrez, José María; León Montero, Guillermo (2017)Adjuvant emulsions are widely used to enhance the antibody response of the animals used as immunoglobulin source for producing antivenoms. Usually, the adjuvant activity of emulsions is attributed both to their ability to ...