Listar Microbiología por autor "47459f3d-d6ca-4094-b546-98c12e8cd7f4"
Mostrando ítems 1-10 de 10
-
A fluorescence-activatable reporter of flavivirus NS2B–NS3 protease activity enables live imaging of infection in single cells and viral plaques
Arias Arias, Jorge Luis; MacPherson, Derek J.; Hill, Maureen E.; Hardy, Jeanne A.; Mora Rodríguez, Rodrigo Antonio (2020-01-09)The genus Flavivirus in the family Flaviviridae comprises many medically important viruses, such as dengue virus (DENV), Zika virus (ZIKV), and yellow fever virus. The quest for thera- peutic targets to combat flavivirus ... -
A Fluorescent Real-Time Plaque Assay Enables Single-Cell Analysis of Virus-Induced Cytopathic Effect by Live-Cell Imaging
Arias Arias, Jorge Luis; Corrales Aguilar, Eugenia; Mora Rodríguez, Rodrigo Antonio (2021-06-22)Conventional plaque assays rely on the use of overlays to restrict viral infection allowing the formation of distinct foci that grow in time as the replication cycle continues leading to countable plaques that are ... -
Dengue Virus Infection of Primary Human Smooth Muscle Cells
Arias Arias, Jorge Luis; Vega Aguilar, Francisco; Corrales Aguilar, Eugenia; Hun Opfer, Ruchilia Laya; Loría Masis, Gilbert David; Mora Rodríguez, Rodrigo Antonio (2018-11-05)Dengue virus (DENV) infection of humans is presently the most important arthropod-borne viral global threat, for which no suitable or reliable animal model exists. Reports addressing the effect of DENV on vascular components ... -
Development and validation of fluorescence live-cell imaging approaches to study flavivirus infection kinetics in animal cells
Arias Arias, Jorge Luis (2021-10-07)The genus Flavivirus in the family Flaviviridae comprises many clinically important viruses, such as dengue virus (DENV), Zika virus (ZIKV), and yellow fever virus (YFV). The quest for therapeutic targets to combat ... -
Fluorescence Imaging Approaches in Flavivirus Research
Arias Arias, Jorge Luis; Mora Rodríguez, Rodrigo Antonio (2021-10-01)The genus Flavivirus within the family Flaviviridae contains many arthropod-borne infectious agents of medical relevance like dengue virus (DENV), Zika virus (ZIKV), and West Nile virus (WNV), among others, that cause ... -
Generation and implementation of reporter BHK-21 cells for live imaging of flavivirus infection
Arias Arias, Jorge Luis; Mora Rodríguez, Rodrigo Antonio (2021-03-05)The genus Flavivirus within the family Flaviviridae includes many viral species of medical importance, such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV), among others. Presently, the identification ... -
In Vitro Inhibition of Zika Virus Replication with Amantadine and Rimantadine Hydrochlorides
Arias Arias, Jorge Luis; Vega Aguilar, Francisco; Picado Soto, Dihalá; Corrales Aguilar, Eugenia; Loría Masís, Gilbert David (2021-09-15)Zika virus (ZIKV) is a mosquito-borne flavivirus in which human infection became relevant during recent outbreaks in Latin America due to its unrecognized association with fetal neurological disorders. Currently, there ... -
MYC dosage compensation is mediated by miRNA-transcription factor interactions in aneuploid cancer
Acón, Man Sai; Geiß, Carsten; Torres Calvo, Jorge; Bravo Estupiñan, Diana; Oviedo Blanco, Guillermo; Arias Arias, Jorge Luis; Rojas Matey, Luis Andres; Báez Villalobos, Edwin; Vásquez Vargas, Gloriana; Oses Vargas, Yendry; Guevara Coto, José Andrés; Segura Castillo, Andrés; Siles Canales, Francisco; Quirós Barrantes, Steve; Régnier Vigouroux, Anne; Mendes, Pedro; Mora Rodríguez, Rodrigo Antonio (2021-12-17)We hypothesize that dosage compensation of critical genes arises from systems- level properties for cancer cells to withstand the negative effects of aneuploidy. We identified several candidate genes in cancer multiomics ... -
Perturbation-Based Modeling Unveils the Autophagic Modulation of Chemosensitivity and Immunogenicity in Breast Cancer Cells
Quirós Fernández, Isaac; Figueroa Protti, Lucía; Arias Arias, Jorge Luis; Brenes Cordero, Norman; Mora Rodríguez, Javier Francisco; Mora Rodríguez, Rodrigo Antonio (2021-09-18)In the absence of new therapeutic strategies, chemotherapeutic drugs are the most widely used strategy against metastatic breast cancer, in spite of eliciting multiple adverse effects and having low responses with an ... -
Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing
Corrales Aguilar, Eugenia; Arias Arias, Jorge Luis; Molina Castro, Silvia Elena; Monturiol Gross, Laura; Lomonte, Bruno (2023-01)SARS-CoV-2 receptor binding domain (RBD) recognizes the angiotensin converting enzyme 2 (ACE2) receptor in host cells that enables infection. Due to its antigenic specificity, RBD production is important for development ...