MARINE RECORD Open Access New records of marine decapods and stomatopods in Área de Conservación Guanacaste (ACG): four years of marine biodiversity inventorying Rita Vargas-Castillo1,3 and Jorge Cortés1,2,3* Abstract The marine area of Área de Conservación Guanacaste (ACG) contains a 43,000 ha formal marine protected area, a 732 ha special management zone in Bahía Santa Elena, and 150 km of wild protected coastline. In an effort to broaden the biodiversity knowledge of all marine taxa present in the area, an inventory was started in 2015 (BioMar-ACG Project). This initiative is being funded by the Guanacaste Dry Forest Conservation Fund (GDFCF) in collaboration with government staff at ACG, and is carried out by Centro de Investigación en Ciencias del Mar y Limnología (CIMAR, Center for Research in Marine Science and Limnology) and Museo de Zoología (Zoology Museum), both from Universidad de Costa Rica (UCR). After four years of the project, 2650 specimens of marine decapod crustaceans and stomatopods have been collected, belonging to 209 species, out of which 99 are new records for ACG, four may be new species and nine (Cyrtoplax panamensis, Glyptoxanthus labyrinthicus, Pachyches marcortezensis, Petrolisthes donadio, Pylopagurus holmesi, Synalpheus pinkfloydi, Typton granulosus, Zenopontonia soror, Neogonodactylus pumilus) are new records for Costa Rica. With this contribution the total number of decapods (257 spp.) and stomatopods (14 spp.) for ACG is 271 species, more than half the species reported for Costa Rica, and more than a quarter of all crustaceans reported for the eastern tropical Pacific. The high concentration of species in ACG may be attributed to the diversity of habitats, the seasonal upwelling and to the recent sampling efforts. In only four years, the BioMar-ACG has increased the number of species in these groups of crustaceans by 37% over the past 85 years of previous studies in the ACG. Keywords: Decapods, Stomatopods, Biodiversity, Inventory, ACG Introduction The first study of marine decapods of Costa Rica was completed by Faxon (1895), and on stomatopods by Schmitt (1940) and Reaka and Manning (1980). Compi- lations of the biodiversity of marine decapod crustaceans and stomatopods in Costa Rica began with a publication by Moran and Dittel (1993), with an annotated list of anomuran and brachyuran crabs from the Pacific and Caribbean coasts. Castro and Vargas (1996) published an annotated list of decapods and stomatopods from Golfo Dulce, while Vargas and Cortés (1997) a list of stomatopods of the country. Afterwards, two other com- pilations on crustaceans were published for Penaeoidea, Sergestoidea, Caridea, Astacidea, Thalassinidea and Palinura in the Caribbean (Vargas and Cortés 1999a) and in the Pacific (Vargas y Cortés 1999b). Vargas and Cortés (2006) published a compilation of the Infraorder Anomura. Some years later, compilations were published in the book “Marine Biodiversity of Costa Rica, Central America” (Wehrtmann and Cortés 2009), which exam- ines all groups of marine organisms reported for Costa Rica. Chapters include all crustacean groups known to be present in this country on the Pacific and Caribbean, including the best-known taxa: stomatopods (35 spp.) © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. * Correspondence: jorge.cortes@ucr.ac.cr 1Museo de Zoología, Universidad de Costa Rica, San Pedro, San José 11501-2060, Costa Rica 2Centro de Investigación en Ciencias del Mar y Limnología (CIMAR), Universidad de Costa Rica, San Pedro, San José 11501-2060, Costa Rica Full list of author information is available at the end of the article Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 https://doi.org/10.1186/s41200-019-0181-6 http://crossmark.crossref.org/dialog/?doi=10.1186/s41200-019-0181-6&domain=pdf http://orcid.org/0000-0003-0561-2121 http://orcid.org/0000-0001-7004-8649 http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/publicdomain/zero/1.0/ mailto:jorge.cortes@ucr.ac.cr (Vargas 2009) and decapods (549 spp.) (Vargas and Wehrtmann 2009). Área de Conservación Guanacaste (ACG) includes sev- eral terrestrial National Parks and Reserves and a Marine Sector that is 43,000 ha and 150 km of protected coast- line (http://www.acguanacaste.ac.cr/acg/que-es-el-acg). It is one of the best-studied conservation areas in Central America, but previous biological research has Table 1 Collection sites at Área de Conservación Guanacaste Code Collection sites Type of environment BEH Bahía El Hachal Intertidal zone BEJ Bajo El Jardín Reef and algae covered coralline rocks BEM Bajo El Machetazo Rocky bottom and small loose rocks BET Bajo El Tigre Rocky bottom and small loose rocks BEV Bajo El Viejón Rocky bottom and small loose rocks BEJ Bajo El Jardín Rocky bottom and small loose rocks BSE Bahía Santa Elena, Pintadero, Playa Cocos Rocky bottom and small loose rocks BLC Bajo Las Chavelas Rocky bottom and small loose rocks BLM Bajo Los Mogotes Rocky bottom and small loose rocks BLR Bajo La Rajada Rocky bottom and small loose rocks BLS Bajo La Salvadita Rocky bottom and small loose rocks BMu Bajo Los Muñecos Rocky bottom and small loose rocks BPi Bajo Pintadero Rocky bottom and small loose rocks BPo Bajo Pochote Rocky bottom and small loose rocks BRo Bajo Rojo Rocky bottom and small loose rocks BTh Bahía Thomas Floating oyster baskets, rocky beach, rocky beach with sponges, live and dead Pocillopora Cua Bahía Cuajiniquil, Playa Cuajiniquil Sand and mud bottoms ICo Isla Cocinera Rocky intertidal zone and reefs IDa Isla David Rocky bottom and small loose rocks IGo Isla Golondrina Pavona clavus reef, dead coral IPe Isla Pelada Rocky intertidal zone, rocks covered with algae ISJ Esquina Conchal, Playa del Maíz, Playa Cactus, Isla San José Rocky intertidal zone and dead Pocillopora ISP Arrecife al NE de Isla San Pedrito, arrecife muerto San Pedrito, San Pedrito Coral reef and dead coral Jun Playa Junquillal, Junquillal esquina norte; Islote Junquillal, Junquillal, Esquina del Tamarindo Rocky intertidal zone, polychaete reef, and Pocillopora Mat Matapalito Coral reef, dead coral and rocky-sandy bottom MCu Manglar en Bahía Cuajiniquil, Manglar 3 Bocas, Manglar 4 × 4 Cuajiniquil Mangrove, mud, rubble with sponges and algae MSE Manglar Santa Elena Mangrove, muddy flats Mue Muelle lado derecho, muelle lado izquierdo, Cástula, Tiza, Hielera Rocky intertidal and subtidal zones, sandy bottom, rubble PCl Piedra Claudio Rocky bottom and small loose rocks with barnacles PCo Playa Corona Rocky intertidal zone PdI Puerta de Iglesia, Isla San José Rocky bottom PGr Playa Potrero Grande, Estero de Potrero Grande Rocky intertidal zone, sandy beach, estuary, mangrove PMa Playa Macaya Rocky intertidal zone, subtidal live coral PPo Punta Pochote Dead corals Pre Playa Respingue Rocky intertidal zone PSe Piedra Seca Rocky bottom and small loose rocks with barnacles 2MM Arrecife 2MM Coral reef Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 Page 2 of 8 http://www.acguanacaste.ac.cr/acg/que-es-el-acg Table 2 New records of marine decapods and stomatopods from Área de Conservación Guanacaste after four years of the BioMar-ACG project. In bold type: new records for Costa Rica Taxon Locality Decapoda Family Albuneidae 1) Albunea lucasia de Saussure, 1853 PGr Family Alpheidae 2) Alpheopsis sp. nov. A BEJ, BTh, ISJ, Cua, Mue, Jun, BLM, MSE 3) Alpheopsis sp. nov. B BEJ, BTh, ISJ, Cua, Mue, Jun, BLM, MSE 4) Alpheus bellimanus Lockington, 1877 BRo, BLM 5) Alpheus bouvieri A. Milne-Edwards, 1878 ISJ, Jun, PCo, Cua, Mue, PRe, Pgr, MSE 6) Alpheus cf. latus Kim & Abele, 1988 MCu 7) Alpheus colombiensis Wicksten, 1988 8) Alpheus cristulifrons Rathbun, 1900 ICo, Jun, ISJ, BEV, BTh, Mue, BRo 9) Alpheus lottini Guérin-Méneville, 1838 [in Guérin-Méneville, 1829–1838] BTh, BEH, IDa, Jun, PMa 10) Alpheus malleator Dana, 1852 BTh, Jun, Mue, Cua, BEV 11) Alpheus saxidomus Holthuis, 1980 2MM, ISJ 12) Alpheus tenuis Kim & Abele, 1988 MCu, PGr, 13) Alpheus websteri Kingsley, 1880 IGo, ISJ, BTh, Cua, BLM, 2MM, BEJ 14) Automate dolichognatha de Man, 1888 Mue, PRe 15) Salmoneus serratidigitus (Coutière, 1898) BTh, Cua 16) Synalpheus cf. lockingtoni Coutière, 1909 BTh 17) Synalpheus mexicanus Coutière, 1909 IGo, ISJ 18) Synalpheus nobilii Coutière, 1909 BTh, Cua, Jun, Mue 19) Synalpheus pinkfloydi Anker, Hultgren & De Grave, 2017 BTh, ISJ, 2MM 20) Synalpheus sanjosei Coutière, 1909 BEH, BTh, ISJ, Mue, PMa, PPo Family Axianassidae 21) Axianassa mineri Boone, 1931 Cua, Mue Family Axiidae 22) Axiopsis baronai Squires, 1977 BRo 23) Neaxius vivesi (Bouvieri, 1895) Mue, ISJ Family Cryptochiridae 24) Opecarcinus crescentus (Edmondson, 1925) BEJ, ICo, Jun Family Diogenidae 25) Calcinus obscurus Stimpson, 1859 BTh, Cua, ICo, ISJ, Jun, PCo, PGr, PMa, PRe 26) Clibanarius albidigitus Nobili, 1901 ICo, Jun, MCu, MSE, Mue, 27) Clibanarius lineatus (H. Milne Edwards, 1848) MCu Family Domeciidae 28) Domecia hispida Eydoux & Souleyet, 1842 Mue Family Epialtidae 29) Acanthonyx petiverii H. Milne Edwards, 1834 BTh, BEM, IPe, 30) Herbstia tumida (Stimpson, 1871) BRo 31) Notolopas lamellatus Stimpson, 1871 MCu 32) Pelia pacifica A. Milne-Edwards, 1875 BTh, Jun, PGr 33) Tyche lamellifrons Bell, 1836 BLM, ISJ, Mue Family Grapsidae Table 2 New records of marine decapods and stomatopods from Área de Conservación Guanacaste after four years of the BioMar-ACG project. In bold type: new records for Costa Rica (Continued) Taxon Locality 34) Goniopsis pulchra (Lockington, 1877) MCu Family Hippolytidae 35) Thor algicola Wicksten, 1987 BTh, BRo, ISJ, IGo, Jun, Cua, PGr 36) Thor sp. nov. PSe Family Hymenoceridae 37) Hymenocera picta Dana, 1852 BTh, 2MM Family Inachidae 38) Coryrhynchus vestitus (Stimpson, 1871) BTh, BEJ, BLM, Cua, Jun, Mue, PCl Family Leucosiidae 39) Persephona townsendi Bell, 1855 ICo 40) Uhlias ellipticus Stimpson, 1871 BTh, ICo, ISJ Family Menippidae 41) Menippe obtusa Stimpson, 1859 Mue Family Ocypodidae 42) Leptuca beebei (Crane, 1941) Cua, MCu, MSE 43) Leptuca musica (Rathbun, 1914) PGr 44) Uca princeps (Smith, 1870) MSE 45) Uca stylifera (H. Milne Edwards, 1852) MSE 46) Ucides occidentalis (Ortmann, 1897) MCu, PGr Family Oziidae 47) Eupilumnus xantusii (Stimpson, 1860) BTh, BEM, ISJ, Mue 48) Ozius perlatus Stimpson, 1860 ICo, Jun Family Paguridae 49) Pagurus nanodes Haig & Harvey, 1991 BTh, IDa, Jun, Mue 50) Phimochirus roseus (Benedict, 1892) BTh 51) Pylopagurus holmesi Schmitt, 1921 ICo, ISP Family Palaemonidae 52) Ascidonia pusilla (Holthius, 1951) BTh 53) Harpiliopsis depressa (Stimpson, 1860) BEH, BTh, IDa, Jun, PMa 54) Palaemon ritteri Holmes 1895 BTh, Cua, ICo, ISJ, Mue, PCo, PRe, PGr 55) Palaemonella holmesi (Nobili, 1907) BTh, BLC, Bro, BEV, ISJ, Mat, Mue 56) Pontonia mexicana Guérin-Méneville, 1855 [in Guérin-Méneville, 1855–1856] BEJ 57) Pseudocoutierea elegans Holthuis, 1951 BET 58) Pseudoveleronia laevifrons (Holthuis, 1951) BET 59) Typton granulosus Ayon-Parente, Hendrickx & Galvan-Villa, 2015 BTh, BEV, Mue, MCu, PGr 60) Zenopontonia soror (Nobili, 1904) BEH, BTh, IPe, Mue Family Panopeidae 61) Acantholobulus mirafloresensis (Abele & Kim, 1989) BTh, BLR, Cua, Mue, MCu, PGr 62) Lophopanopeus maculatus Rathbun, 1898 ISJ 63) Panopeus purpureus Lockington, 1877 BTh, Cua, MCu, MSE, PGr 64) Prionoplax ciliata Smith, 1870 MCu, MSE, PGr Family Parthenopidae 65) Heterocrypta colombiana Garth, 1940 Cua, Mue Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 Page 3 of 8 focused primarily on the terrestrial part. In order to ex- pand the knowledge of marine biodiversity in this re- gion, the project entitled “Marine Biodiversity of Área de Conservación Guanacaste” (BioMar-ACG) was launched in 2015. The project has been funded by the Guanacaste Dry Forest Conservation Fund (GDFCF), and carried out by the Centro de Investigación en Ciencias del Mar y Limnología (CIMAR) and Museo de Zoología, both from Universidad de Costa Rica (UCR) (Cortés & Joyce in prep). The project has also received necessary support from the government staff of ACG, local marine “paratax- onomists” supported by GDFCF and private foundations. Following the compilation on marine biodiversity in ACG published by Cortés (2017), this paper presents an update of the inventory of marine decapod crustaceans and stomatopods, with new records for ACG as well as new records for the country. This is the result of work from mid 2015 to early 2019 by the BioMar-ACG project. Methodology Sixty sites were visited in ACG, from Punta Descartes to Islas Murciélago (Table 1), including rocky beaches (most common) and muddy beaches, shallow and deep reefs, shallow and deep rocky areas, estuaries, mangroves and areas deeper than 45 m. The location, geographic coordinates, depth and substrate type were registered. In the rocky beaches during low tide, rocks were lifted to collect the organisms that live beneath them and the substrate was dug up to collect the organisms that live buried in the sediments. In the muddy beach areas, organisms were also collected from the mud. To collect the organisms that live within coral reefs, an indirect method was used: dead coral rocks were brought to the surface and broken apart to collect the organisms that mutually live inside. A similar method was used areas of rocky ocean floor, where collected specimens were brought to the surface. In the mangroves, specimens Table 2 New records of marine decapods and stomatopods from Área de Conservación Guanacaste after four years of the BioMar-ACG project. In bold type: new records for Costa Rica (Continued) Taxon Locality 66) Piloslambrus triangulus (Stimpson, 1860) BTh Family Penaeidae 67) Penaeus stylirostris Stimpson, 1871 BTh 68) Penaeus vannamei Boone, 1931 BTh Family Pilumnidae 69) Pilumnus limosus Smith, 1869 BTh, BEV, MCu, Mue 70) Pilumnus stimpsoni Miers, 1886 BLM, BRo, BLS, BEV, IGo, ISP, Mue, PSe Family Pinnotheridae 71) Austinixa sp. indet Jun 72) Pinnixa sp. indet Mue 73) Pinnotheres sp. indet PGr 74) Tumidotheres sp. indet BTh Family Plagusiidae 75) Plagusia immaculata Lamarck, 1818 BTh, PRe, PGr Family Porcellanidae 76) Megalobrachium erosum (Glassell, 1936) BTh, Cua 77) Neopisosoma mexicanum (Streets, 1871) Jun, PGr 78) Pachycheles calculosus Haig, 1960 Jun 79) Pachycheles marcortezensis Glassell, 1936 BTh 80) Pachycheles spinidactylus Haig, 1957 ISJ, Jun, 2MM 81) Petrolisthes donadio Hiller & Werding, 2007 BTh, BLM, IGo, ISJ, ISP, Jun, Mat, Mue, Pse, PPo 82) Polyonyx confinis Haig, 1960 Jun, Mue 83) Ulloaia perpusillia Glassell, 1938 BLR Family Portunidae 84) Achelous tuberculatus Stimpson, 1860 BTh, ISJ, MSE Family Processidae 85) Processa peruviana Wicksten, 1983 Cua, ISJ, Mue Family Pseudorhombilidae 86) Cyrtoplax panamensis Ziesenhenne in Garth, 1940 MSE Familia Raninidae 87) Raninoides benedicti Rathbun, 1935 PGr Family Rhynchocinetidae 88) Cinetorhynchus sp. nov. BTh Family Sesarmidae 89) Sesarma sulcatum Smith, 1870 MCu Family Trapeziidae 90) Trapezia digitalis Latreille, 1828 BEH, BTh, IDa, Jun, Mat, PMa Family Upogebiidae 91) Upogebia thistlei Williams, 1986 BTh, BSE,Jun, Mue Family Xanthidae 92) Glyptoxanthus labyrinthicus (Stimpson, 1860) BLS 93) Lipaesthesius leeanus Rathbun, 1898 BEJ, BEM, BEV, BLM, BPo, PCl, 94) Lipkemedaeus spinulifer (Rathbun, 1898) BTh, BEM, BEV, BLR, BLS, BPi, BRo, PSe Table 2 New records of marine decapods and stomatopods from Área de Conservación Guanacaste after four years of the BioMar-ACG project. In bold type: new records for Costa Rica (Continued) Taxon Locality 95) Platypodiella rotundata (Stimpson, 1860) BTh, BEM, BRo, Cua, ICo, IGo, ISJ, Jun, MCu, Mue, PGr Stomatopoda Family Gonodactylidae 96) Neogonodactylus pumilus (Manning, 1970) ISJ, 97) Neogonodactylus stanschi (Schmitt, 1940) BEV, Mat Family Squillidae 98) Cloridopsis dubia (H. Milne Edwards, 1837) BTh 99) Meiosquilla dawsoni Manning, 1970 MSE Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 Page 4 of 8 were collected during walks in the forest and intertidal zone, and they were taken from the roots and ground. Using a shovel, the substrate was dug up and sifted to look for those organisms that live buried in the mud. In the external part of the mangrove, a similar process was followed, but also fallen leaves and tree trunks were ex- amined for organisms. Deep dredging sampling (down to 50 m depth) was carried out with a Van Veen dredge with a 25 × 25 cm opening. Other organisms were manually collected during dives, and some samples of crustaceans were associated with octocorals. Collected specimens were placed in plastic containers with a net and then submerged in a bucket filled with seawater. Afterwards, the organisms were photographed in an improvised lab, a code was assigned to each speci- men, a preliminary identification was provided and a sample of tissue was taken for barcoding. Once this process was finished, the organisms were sent to the Fig. 1 New records of crustaceans for Costa Rica, with their BioMar-ACG sample code: (a) Cyrtoplax panamensis,, 17-BMACGRV-02644-ACG001862; (b) Glyptoxanthus labyrinthicus, 16-BMACGRV-02104-ACG005676; (c) Neogonodactylus pumilus, 16-BMACGRV-02407-ACG006086; (d) Pachyches marcortezensis, 15-BMACGRV-00507-ACG006967; (e) Petrolisthes donadio, 16-BMACGRV-02177-ACG009006; (f) Pylopagurus holmesi, 16-BMACGRV- 02414-ACG007167; (g) Synalpheus pinkfloydi, 16-BMACGRV-02245-ACG003908; (h) Typton granulosus, 16-BMACGRV-01941-ACG001783, and (i) Zenopontonia soror, 15-BMACGRV-00590-ACG009037 Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 Page 5 of 8 Zoology Museum at Universidad de Costa Rica, where a definite identification was provided and the samples were catalogued and stored. The names of the species used are as in WoRMS (http://www.marinespecies.org). Results and discussion By early 2019, 2650 specimens have been collected (Table 2), corresponding to 209 species, 99 of which are new records for ACG, four could be new undescribed species, and nine are also new records for Costa Rica: Cyrtoplax panamensis (Fig. 1a), Glyptoxanthus labyr- inthicus (Fig. 1b), Neogonodactylus pumilus (Fig. 1c), Pachyches marcortezensis (Fig. 1d), Petrolisthes donadio (Fig. 1e), Pylopagurus holmesi (Fig. 1f), Synalpheus pinkfloydi (Fig. 1g), Typton granulosus (Fig. 1h) and Zenopontonia soror (Fig. 1i). Cortés (2017) reported 172 species from ACG, 162 decapods and 10 stomato- pods. In this study 209 species have been collected so far, 200 decapods and 9 stomatopods. Of the decapod species reported by Cortés (2017), 57 species of deca- pods and 5 species of stomatopod have not been collected yet. These belong mainly to organisms com- monly found at depths greater than 40 m, that were collected by dredging. Taking this into account, the total number of species found in the area is 271, which includes 257 decapods and 14 stomatopods. During the last four years since the BioMar-ACG project started, 99 new records have been added to the known species from ACG (Table 2) This represents an increase of 37% over the almost 85 years of previous studies in the region. The number of species of decapods and stomatopods reported for ACG (271) is the highest number found in Costa Rica and among the highest reported in the east- ern tropical Pacific ETP (Table 3). The ETP extends from the Gulf of California to southern Ecuador and include several oceanic islands (Robertson and Kramer 2009). ACG has more than half the species reported for Costa Rica and about a quarter of decapods and sto- matopods crustaceans reported for the ETP (Boschi 2000; Cortés et al. 2017) (Table 3). The high diversity of decapods and stomatopods at ACG may be due to the diversity of habitats: beaches of different wave and sediment regimes, islands and continental shorelines, mangrove forests, seagrass beds, coral reefs (live and dead), intertidal and submerged rocky platforms, sandy and muddy bottoms and deep areas (Cortés 2017). The region is exposed to season upwelling, with temperatures ranging from 15° to 30 °C (Cortés et al. 2014). And fi- nally, the sampling effort makes a difference in how many species are reported from an area, as demon- strated in this paper. Up to now 60 sites have been sampled, with many more still to explore, such as the coastal area of Penín- sula Santa Elena, south of Playa Naranjo and offshore, as well as some habitats such as sandy beaches, and deep rocky and muddy bottoms. Definitely more species of decapods and stomatopods will be found increasing the biodiversity of crustaceans in Área de Conservación de Guanacaste. Resumen El sector marino del Area de Conservación Guanacaste (ACG) posee 43,000 ha y 150 km de costa, en su mayoría poco estudiada. En un esfuerzo por conocer mejor la biodiversidad de todos los taxones marinos presentes en el área, se inicio en el 2015 un inventario (Proyecto BioMar-ACG). Esta iniciativa está siendo sustentada por la Guanacaste Dry Forest Conservation Fund (GDFCF) y desarrollada por el Centro de Investigación en Ciencias del Mar y Limnología (CIMAR) y el Museo de Zoología, ambos de la Universidad de Costa Rica (UCR). Table 3 Richness of stomatopods and decapods in the eastern tropical Pacific (ETP) Stomatopoda Decapoda Total References México 28 1029a Hendrickx 2005a, 2005b; M.E. Hendrickx, personal communication, 2019 Eastern Pacific 53 825b 878 Boschi 2000; Cortés et al. 2017; Salgado-Barragán & Hendrickx 2010 Costa Rica 29 437 466 Vargas 2009; Vargas & Wehrtmann 2009 Colombia 11 378 389 Lemaitre & Álvarez-León 1992; López & Jaimes 2014 ACG, Costa Rica 14 257 271 This study Oaxaca, México 15 197 212 Bastida-Zavala et al. 2013 Galápagos, Ecuador 5 205 210 Hickman & Zimmermn 2000 Clipperton, France 4 190 194 Poupin et al. 2009 Isla del Coco, Costa Rica 6 139 145 Vargas-Castillo & Wehrtmann 2008; Cortés 2012 Bahía Culebra, Costa Rica 3 99 102 Cortés et al. 2012 Golfo Dulce, Costa Rica 2 71 71 Morales-Ramírez 2011; Castro & Vargas 1996 aIncludes pelagic and deep and shallow water species (M.E. Hendrickx, personal communication, 2019) bIncludes only shallow water species Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 Page 6 of 8 http://www.marinespecies.org Transcurridos cuatro años del proyecto, se han recolec- tado 2650 especímenes de crustáceos decápodos y esto- matópodos marinos pertenecientes a 209 especies, de las cuales 99 son nuevas para el ACG, cuatro podrían ser nuevas especies para la ciencia y nueve (Cyrtoplax pana- mensis, Glyptoxanthus labyrinthicus, Pachyches marcorte- zensis, Petrolisthes donadio, Pylopagurus holmesi, Synalpheus pinkfloydi, Typton granulosus, Zenopontonia soror y Neogonodactylus pumilus) son ampliaciones de ámbito y nuevos informes para el país. Con esta contribu- ción aumenta el número de decápodos a 257 spp. y de estomatópodos a 14 spp. para un total de 271 spp. para ACG. Esto es más de la mitad las especies conocidas de estos grupos para Costa Rica y más una cuarta parte de las especies conocidas para el Pacífico Tropical Oriental. En cuatro años el proyecto BioMar-ACG ha aumentado el número de especies de decápodos y estomatópodos del ACG en 37% más que lo que se conocía a partir de estu- dios en los últimos 85 años. Conclusions The model for marine biodiversity inventory developed with the BioMar-ACG project, the partnership between government, academia, private funding and local para- taxonomists, accelerates the rate of species discovery and reporting. It also makes species information and project results available in an open access format. In only for years of the project the number of decapods and stomatopods of ACG increased by 37% over the past 85 years of previus studies, for a total of 271 species. This number represnets more than half the species re- ported for Costa Rica, and more than a quarter of all decapods and stomatopods reported for the eastern tropical Pacific. Acknowledgements The authors acknowledge and thank the following people and organizations for their efforts and significant support to the BioMar-ACG project: Daniel Janzen, Winnie Hallwachs, Frank Joyce, María Marta Chavarría, Roger Blanco, Eric Palola, Yelba Vega, Gilberth Ampie, Diving Center Cuajiniquil, the Santa Elena Lodge, the Guanacaste Dry Forest Conservation Fund, the Wege Foundation, the New England BioLabs Foundation, the Wallace Genetic Foundation and the Centre for Biodiversity Genomics, University of Guelph. We thank Francilena Carranza for preparing the photographs and Eric Palola and anonymous reviewers for the review of the manuscript. Michel E. Hendrickx was very helpful in the preparation of the reviewed draft of the paper. Authors’ contributions RV collected and identified the specimens, wrote a first draft of the manuscript. JC, conceived the main project, helped with logistics and obtaining the funds, wrote some sections of the paper, helped prepared the images and the final draft of the paper. All authors read and approved the final manuscript. Authors’ information Rita Vargas: Collection Manager and curator of Crustaceans of the Zoology Museum of the University of Costa Rica. Jorge Cortés: Senior researcher at the Center for Research in Marine Science and Limnology, and professor at the School of Biology, both at the University of Costa Rica. Funding Funding source indicated in the Acknowledgement section. Availability of data and materials Data will be made available in the project website. The specimens are deposited in the Museo de Zoología, Universidad de Costa Rica, and can be checked there. Ethics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests. Author details 1Museo de Zoología, Universidad de Costa Rica, San Pedro, San José 11501-2060, Costa Rica. 2Centro de Investigación en Ciencias del Mar y Limnología (CIMAR), Universidad de Costa Rica, San Pedro, San José 11501-2060, Costa Rica. 3Escuela de Biología, Universidad de Costa Rica, San Pedro, San José 11501-2060, Costa Rica. Received: 28 July 2019 Accepted: 28 October 2019 References Bastida-Zavala JR, del S G-MM, Rosas-Alquicira EF, López-Pérez RA, Benítez- Villalobos F, Meraz-Hernando JF, Torres-Huerta AM, Montoya-Márquez A, Barrientos-Luján NA. Marine and coastal biodiversity of Oaxaca, Mexico. Check List. 2013;9:329–90. Boschi E. Species of decapod crustaceans and their distribution in the American marine zoogeographic provinces. Rev Invest Des Pesq. 2000;13:1–63. Castro M, Vargas R. Annotated list of species of marine crustaceans (Decapoda and Stomatopoda) from Golfo Dulce, Costa Rica. 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Vargas-Castillo and Cortés Marine Biodiversity Records (2019) 12:21 Page 8 of 8 https://doi.org/10.1007/978-1-4020-8278-8_17 https://doi.org/10.15517/rbt.v54i2.13894 https://doi.org/10.15517/rbt.v54i2.13894 https://doi.org/10.1007/978-1-4020-8278-8_19 https://doi.org/10.1007/978-1-4020-8278-8_19 Abstract Introduction Methodology Results and discussion Resumen Conclusions Acknowledgements Authors’ contributions Authors’ information Funding Availability of data and materials Ethics approval and consent to participate Consent for publication Competing interests Author details References Publisher’s Note