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dc.creatorRetana Moreira, Lissette
dc.creatorCornet Gómez, Alberto
dc.creatorSepúlveda Justo, María del Rosario
dc.creatorMolina Castro, Silvia Elena
dc.creatorAlvarado Ocampo, Johan
dc.creatorChaves Monge, Frida
dc.creatorJara Rojas, Mariana
dc.creatorOsuna Carrillo de Albornoz, Antonio
dc.creatorAbrahams Sandí, Elizabeth
dc.date.accessioned2024-02-29T20:59:01Z
dc.date.available2024-02-29T20:59:01Z
dc.date.issued2024
dc.identifier.citationhttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1346021/fulles_ES
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/10669/90997
dc.description.abstractNaegleria fowleri is the causative agent of primary amoebic meningoencephalitis, a rapid and acute infection of the central nervous system with a fatal outcome in >97% of cases. Due to the infrequent report of cases and diagnostic gaps that hinder the possibility of recovering clinic isolates, studies related to pathogenesis of the disease are scarce. However, the secretion of cytolytic molecules has been proposed as a factor involved in the progression of the infection. Several of these molecules could be included in extracellular vesicles (EVs), making them potential virulence factors and even modulators of the immune response in this infection. In this work, we evaluated the immunomodulatory effect of EVs secreted by two clinic isolates of Naegleria fowleri using in vitro models. For this purpose, characterization analyses between EVs produced by both isolates were first performed, for subsequent gene transcription analyses post incubation of these vesicles with primary cultures from mouse cell microglia and BV-2 cells. Analyses of morphological changes induced in primary culture microglia cells by the vesicles were also included, as well as the determination of the presence of nucleic acids of N. fowleri in the EV fractions. Results revealed increased expression of NOS, proinflammatory cytokines IL-6, TNF-α, and IL-23, and the regulatory cytokine IL-10 in primary cultures of microglia, as well as increased expression of NOS and IL-13 in BV-2 cells. Morphologic changes from homeostatic microglia, with small cellular body and long processes to a more amoeboid morphology were also observed after the incubation of these cells with EVs. Regarding the presence of nucleic acids, specific Naegleria fowleri DNA that could be amplified using both conventional and qPCR was confirmed in the EV fractions. Altogether, these results confirm the immunomodulatory effects of EVs of Naegleria fowleri over microglial cells and suggest a potential role of these vesicles as biomarkers of primary acute meningoencephalitis.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-C1-061]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-C2-600]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourceFrontiers in Microbiology, vol.15, pp.1-16.es_ES
dc.subjectCELLSes_ES
dc.subjectGENESes_ES
dc.subjectMICROGLIAes_ES
dc.subjectVESICLESes_ES
dc.titleProviding an in vitro depiction of microglial cells challenged with immunostimulatory extracellular vesicles of Naegleria fowleries_ES
dc.typeartículo originales_ES
dc.identifier.doi10.3389/fmicb.2024.1346021
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.identifier.codproyecto803-C2-600
dc.identifier.codproyecto803-C1-061


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