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dc.creatorEscudero Leyva, Efraín
dc.creatorQuirós Guerrero, Luis Manuel
dc.creatorVásquez Chaves, Víctor
dc.creatorPereira Reyes, Reinaldo
dc.creatorChaverri Echandi, Priscila
dc.creatorTamayo Castillo, Giselle
dc.date.accessioned2023-08-29T16:48:01Z
dc.date.available2023-08-29T16:48:01Z
dc.date.issued2023
dc.identifier.issn2470-1343
dc.identifier.urihttps://hdl.handle.net/10669/89926
dc.description.abstractFungi exhibit a wide range of ecological guilds, but those that live within the inner tissues of plants (a.k.a. endophytes) are particularly relevant due to the benefits they sometimes provide to their hosts, such as herbivory deterrence, disease protection, and growth promotion. Recently, endophytes have gained interest as potential biocontrol agents against crop pathogens, for example, coffee plants (Coffea arabica). Published results from research done in our laboratory showed that endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the effects of the American Leaf Spot of Coffee (Mycena citricolor). One of these isolates (GU11N) from the plant Randia grandifolia was identified as Daldinia eschscholtzii (Xylariales). Its antagonism mechanisms, effects, and chemistry against M. citricolor were investigated through the analysis of its volatile profile alone and in the presence of the pathogen in contactless and dual culture assays. The experimental design involved direct sampling of agar plugs in vials for headspace (HS) and headspacesolid- phase-microextraction (HS-SPME) Gas Chromatography Mass Spectrometry (GC-MS) analysis. Additionally, we used UHPLC-HRMS/MS to identify non-volatile compounds from organic extracts of the mycelia involved in the interaction. Results showed that more volatile compounds were identified using HS-SPME (39 components) than with the HS technique (13 components), sharing only 12 compounds. Statistical tests suggest that D. eschscholtzii inhibited the growth of M. citricolor through the release of VOCs containing a combination of 1,8- dimethoxynapththalene and terpene compounds affecting the M. citricolor pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated in an in vitro test against M. citricolor pseudopilei; SEM photographs confirmed the structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of fatty acid derivatives was found among the putatively identified compounds, however, a considerable proportion of features (37.3%) remained unannotated. In conclusion, our study suggests that D. eschscholtzii has a potential as a biocontrol agent against M. citricolor and that 1,8-dimethoxynaphthalene contributes to the observed damage to the pathogen’s reproductive structures.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[809-B7-176]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[809-C1-604]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourceACS Omega 2023, 8, 31373–31388es_ES
dc.subjectFUNGIes_ES
dc.subjectCOFFE PLANTSes_ES
dc.subjectPATHOLOGYes_ES
dc.subjectORGANIC COMPOUNDSes_ES
dc.titleDifferential volatile organic compound expression in the interaction of Daldinia eschscholtzii and Mycena citricolores_ES
dc.typeartículo originales_ES
dc.identifier.doi10.1021/acsomega.3c03865
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.identifier.codproyecto809-B7-176
dc.identifier.codproyecto809-C1-604


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional