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dc.creatorApú Leitón, Navilla
dc.creatorMadrigal Redondo, German
dc.creatorHerrera Vega, María
dc.creatorCorrales Aguilar, Eugenia
dc.creatorSegura Ulate, Ismael
dc.date.accessioned2023-08-11T14:38:31Z
dc.date.available2023-08-11T14:38:31Z
dc.date.issued2023-02
dc.identifier.issn2391-5412
dc.identifier.urihttps://hdl.handle.net/10669/89858
dc.description.abstractMost laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use ELISA or CLIA; however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests are a low-cost, equipment-free option, but their high price may make them less suitable for conducting seroprevalence surveys. Here, we describe a simple dual antigen ELISA dot-blot test to detect anti-SARS-CoV-2 IgG antibodies with high sensitivity (94 to 98%) and specificity (92 to 100%), compared to commercially available ELISA and CLIA options. Additionally, this ELISA dot-blot test can be completed in one hour using minimal laboratory equipment. Importantly, this immunoassay is significantly more affordable than most LFIA tests available on the global market. The dot-blot strips may be stored for up to 7 days under freezing conditions. This ELISA dot-blot test is a cost-effective option for conducting seroprevalence screenings in areas lacking ELISA or CLIA facilities, compared to LFIA tests.es_ES
dc.language.isoenges_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourceOpen Life Scienceses_ES
dc.subjectSARS-CoV-2es_ES
dc.subjectCOVID-19es_ES
dc.subjectantibodieses_ES
dc.subjectseroprevalencees_ES
dc.subjectELISA dot-blotes_ES
dc.subjectIMMUNOLOGYes_ES
dc.titleDevelopment of an instrument-free and low-cost ELISA dot-blot test to detect antibodies against SARS-CoV-2es_ES
dc.typeartículo originales_ES
dc.identifier.doi10.1515/biol-2022-0577
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones Farmacéuticas (INIFAR)es_ES
dc.identifier.codproyecto803-C0-334


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Atribución 4.0 Internacional
Except where otherwise noted, this item's license is described as Atribución 4.0 Internacional