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Helicobacter pylori infection induces gastric precancerous lesions and persistent expression of Angpt2, Vegf-A and Tnf-A in a mouse model
dc.creator | Malespín Bendaña, Wendy Karina | |
dc.creator | Alpízar Alpízar, Warner | |
dc.creator | Figueroa Protti, Lucía | |
dc.creator | Reyes Moreno, Ledis | |
dc.creator | Molina Castro, Silvia Elena | |
dc.creator | Une, Clas Allan | |
dc.creator | Ramírez Mayorga, Vanessa | |
dc.date.accessioned | 2023-02-21T14:43:07Z | |
dc.date.available | 2023-02-21T14:43:07Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2234-943X | |
dc.identifier.uri | https://hdl.handle.net/10669/88205 | |
dc.description.abstract | Helicobacter pylori colonizes the gastric mucosa and induces chronic inflammation. Using a mouse model of H. pylori-induced gastritis, we evaluated the mRNA and protein expression levels of proinflammatory and proangiogenic factors, as well as the histopathological changes in gastric mucosa in response to infection. Five- to six-week-old female C57BL/6N mice were challenged with H. pylori SS1 strain. Animals were euthanized after 5-, 10-, 20-, 30-, 40- and 50- weeks post infection. mRNA and protein expression of Angpt1, Angpt2, VegfA, Tnfa, bacterial colonization, inflammatory response and gastric lesions were evaluated. A robust bacterial colonization was observed in 30 to 50 weeksinfected mice, which was accompanied by immune cell infiltration in the gastric mucosa. Compared to non-infected animals, H. pylori-colonized animals showed an upregulation in the expression of Tnf-A, Angpt2 and VegfA at the mRNA and protein levels. In contrast, Angpt1 mRNA and protein expression was downregulated in H. pylori-colonized mice. Our data show that H. pylori infection induces the expression of Angpt2, Tnf-A and Vegf-A in murine gastric epithelium. This may contribute to the pathogenesis of H. pylori-associated gastritis, however the significance of this should be further addressed | es_ES |
dc.description.sponsorship | Ministerio de Ciencia y Tecnología, Gobierno de Costa Rica//CONICIT/Costa Rica | es_ES |
dc.language.iso | eng | es_ES |
dc.source | Frontiers in Oncology 13, 2023 | es_ES |
dc.subject | Helicobacter pylori | es_ES |
dc.subject | angiogénesis | es_ES |
dc.subject | Modelo animal | es_ES |
dc.subject | INFECTIOUS DISEASES | es_ES |
dc.title | Helicobacter pylori infection induces gastric precancerous lesions and persistent expression of Angpt2, Vegf-A and Tnf-A in a mouse model | es_ES |
dc.type | artículo original | es_ES |
dc.identifier.doi | 10.3389/fonc.2023.1072802 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Laboratorio de Ensayos Biológicos (LEBI) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Estructuras Microscópicas (CIEMIC) | es_ES |
dc.identifier.codproyecto | 742-B7-A02 |