Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection.
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Date
2021Author
Castro Córdova, Pablo
Mora Uribe, Paola
Reyes Ramírez, Rodrigo
Cofré Araneda, Glenda
Orozco Aguilar, Josué
Brito Silva, Christian
Mendoza León, María José
Kuehne, Sarah Anna
Minton, Nigel Peter
Pizarro Guajardo, Marjorie
Paredes Sabja, Daniel
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Clostridioides difficile spores produced during infection are important for the recurrence of the
disease. Here, we show that C. difficile spores gain entry into the intestinal mucosa via
pathways dependent on host fibronectin-α5β1 and vitronectin-αvβ1. The exosporium protein
BclA3, on the spore surface, is required for both entry pathways. Deletion of the bclA3 gene in
C. difficile, or pharmacological inhibition of endocytosis using nystatin, leads to reduced entry
into the intestinal mucosa and reduced recurrence of the disease in a mouse model. Our
findings indicate that C. difficile spore entry into the intestinal barrier can contribute to spore
persistence and infection recurrence, and suggest potential avenues for new therapies.
External link to the item
10.1038/s41467-021-21355-5Collections
- Farmacia [172]