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dc.creatorQuirós Fernández, Isaac
dc.creatorFigueroa Protti, Lucía
dc.creatorArias Arias, Jorge Luis
dc.creatorBrenes Cordero, Norman
dc.creatorMora Rodríguez, Javier Francisco
dc.creatorMora Rodríguez, Rodrigo Antonio
dc.date.accessioned2022-07-20T14:05:57Z
dc.date.available2022-07-20T14:05:57Z
dc.date.issued2021-09-18
dc.identifier.citationhttps://www.mdpi.com/journal/metaboliteses_ES
dc.identifier.issn2218-1989
dc.identifier.urihttps://hdl.handle.net/10669/86989
dc.description.abstractIn the absence of new therapeutic strategies, chemotherapeutic drugs are the most widely used strategy against metastatic breast cancer, in spite of eliciting multiple adverse effects and having low responses with an average 5-year patient survival rate. Among the new therapeutic targets that are currently in clinical trials, here, we addressed the association between the regulation of the metabolic process of autophagy and the exposure of damage-associated molecular patterns associated (DAMPs) to immunogenic cell death (ICD), which has not been previously studied. After validating an mCHR-GFP tandem LC3 sensor capacity to report dynamic changes of the autophagic metabolic flux in response to external stimuli and demonstrating that both basal autophagy levels and response to diverse autophagy regulators fluctuate among different cell lines, we explored the interaction between autophagy modulators and chemotherapeutic agents in regards of cytotoxicity and ICD using three different breast cancer cell lines. Since these interactions are very complex and variable throughout different cell lines, we designed a perturbation-based model in which we propose specific modes of action of chemotherapeutic agents on the autophagic flux and the corresponding strategies of modulation to enhance the response to chemotherapy. Our results point towards a promising therapeutic potential of the metabolic regulation of autophagy to overcome chemotherapy resistance by eliciting ICD.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B6-600]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourceMetabolites, 11, 2021es_ES
dc.subjectautophagyes_ES
dc.subjectimmunogenic cell deathes_ES
dc.subjectchemotherapyes_ES
dc.subjectperturbation-based modelinges_ES
dc.titlePerturbation-Based Modeling Unveils the Autophagic Modulation of Chemosensitivity and Immunogenicity in Breast Cancer Cellses_ES
dc.typeartículo científicoes_ES
dc.identifier.doihttps://doi.org/10.3390/metabo11090637
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.codproyecto803-B6-600


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