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dc.creatorRetana Moreira, Lissette
dc.creatorSteller Espinoza, María Fernanda
dc.creatorChacón Camacho, Natalia
dc.creatorCornet Gómez, Alberto
dc.creatorSáenz Arce, Giovanni
dc.creatorOsuna Carrillo de Albornoz, Antonio
dc.creatorLomonte, Bruno
dc.creatorAbrahams Sandí, Elizabeth
dc.description.abstractAbstract: Extracellular vesicles (EVs) are small lipid vesicles released by both prokaryotic and eu-karyotic cells, involved in intercellular communication, immunomodulation and pathogenesis. In this study, we performed a characterization of the EVs produced by trophozoites of a clinical isolate of the free-living amoeba Naegleria fowleri (N. fowleri). Size distribution, zeta potential, protein profile and protease activity were analyzed. Under our incubation conditions, EVs of different sizes were observed, with a predominant population ranging from 206 to 227 nm. SDS-PAGE revealed protein bands of 25 to 260 KDa. The presence of antigenic proteins was confirmed by Western blot, which evidenced the strongest recognition by rat polyclonal antibodies raised against N. fowleri in the region close to 80 KDa and included peptidases, as revealed by zymography. Proteins in the selected immunorecognized bands were further identified using nano-ESI-MS/MS. A preliminary proteomic profile of the EVs identified at least 184 proteins as part of the vesicles’ cargo. Protease activity assays, in combination with the use of inhibitors, revealed the predominance of serine proteases. The present characterization uncovers the complexity of EVs produced by N. fowleri, suggesting their potential relevance in the release of virulence factors involved in pathogenicity. Owing to their cargo’s diversity, further research on EVs could reveal new therapeutic targets or biomarkers for developing rapid and accurate diagnostic tools for lethal infections, such as the one caused by this amoeba.es_ES
dc.description.sponsorshipVicerrectoría de Investigación/[803-C1-061]/VI/Costa Ricaes_ES
dc.description.sponsorshipVicerrectoría de Investigación/[803-C2-600]/VI/Costa Ricaes_ES
dc.sourceBiology; Vol. 11: 2022es_ES
dc.subjectExtracellular vesicleses_ES
dc.subjectNaegleria fowleries_ES
dc.titleCharacterization of Extracellular Vesicles Secreted by a Clinical Isolate of Naegleria fowleri and Identification of Immunogenic Components within Their Protein Cargoes_ES
dc.typeartículo científicoes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES

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