Show simple item record

dc.creatorOrtiz Chaves, Natalia
dc.creatorDelgado Carazo, Juan Carlos
dc.creatorDíaz Oreiro, Cecilia
dc.date.accessioned2022-05-06T16:59:38Z
dc.date.available2022-05-06T16:59:38Z
dc.date.issued2021
dc.identifier.citationhttps://www.dovepress.com/importance-of-mevalonate-pathway-lipids-on-the-growth-and-survival-of--peer-reviewed-fulltext-article-CEGes_ES
dc.identifier.issn1178-7023
dc.identifier.urihttps://hdl.handle.net/10669/86550
dc.description.abstractPurpose: This preclinical study aims to determine the effect of drugs that alter isoprenoids and cholesterol metabolism in the homeostasis of gastric carcinoma cell lines in the search for new therapeutic targets for stomach cancer. Materials and methods: Primary (AGS) and metastatic (NCI-N87) gastric cancer cell lines were treated with simvastatin and terbinafine, two inhibitors of the mevalonate pathway, and avasimibe, an inhibitor of cholesterol esterification. Cell viability and growth were measured as well as cholesterol levels and the expression of the hydroxy methyl-glutaryl CoA reductase (HMGCR) and the LDL receptor (LDLR). Results: Primary and metastatic gastric carcinoma cells show different sensitivity to drugs that affect isoprenoid synthesis and the metabolism and uptake of cholesterol. Isoprenoids are involved in the growth and viability of both types of cells, but the role of free and esterified cholesterol for metastatic gastric cell survival is not as evident as for primary gastric cancer cells. Differential expression of LDLR due to mevalonate pathway inhibition suggests variations in the regulation of cholesterol uptake between primary and metastatic cancer cells. Conclusion: These results indicate that at least for primary gastric cancer, statins and avasimibe are promising candidates as potential novel antitumor drugs that target the metabolism of isoprenoids and cholesterol of gastric tumors.es_ES
dc.description.abstractPurpose: This preclinical study aims to determine the effect of drugs that alter isoprenoids and cholesterol metabolism in the homeostasis of gastric carcinoma cell lines in the search for new therapeutic targets for stomach cancer. Materials and Methods: Primary (AGS) and metastatic (NCI-N87) gastric cancer cell lines were treated with simvastatin and terbinafine, two inhibitors of the mevalonate pathway, and avasimibe, an inhibitor of cholesterol esterification. Cell viability and growth were measured as well as cholesterol levels and the expression of the hydroxy methyl-glutaryl CoA reductase (HMGCR) and the LDL receptor (LDLR). Results: Primary and metastatic gastric carcinoma cells show different sensitivity to drugs that affect isoprenoid synthesis and the metabolism and uptake of cholesterol. Isoprenoids are involved in the growth and viability of both types of cells, but the role of free and esterified cholesterol for metastatic gastric cell survival is not as evident as for primary gastric cancer cells. Differential expression of LDLR due to mevalonate pathway inhibition suggests variations in the regulation of cholesterol uptake between primary and metastatic cancer cells. Conclusion: These results indicate that at least for primary gastric cancer, statins and avasimibe are promising candidates as potential novel antitumor drugs that target the metabolism of isoprenoids and cholesterol of gastric tumors.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[422-B7-098]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourceClinical and Experimental Gastroenterology, vol.14, pp.217-228.es_ES
dc.subjectCholesteroles_ES
dc.subjectIsoprenoidses_ES
dc.subjectGastric canceres_ES
dc.subjectMetastasises_ES
dc.subjectSimvastatines_ES
dc.subjectTerbinafinees_ES
dc.subjectAvasimibees_ES
dc.titleImportance of mevalonate pathway lipids on the growth and survival of primary and metastatic gastric carcinoma cellses_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.2147/CEG.S310235
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto422-B7-098


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record