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dc.creatorWu Wu, Jannette Wen Fang
dc.creatorSolano Redondo, Óscar Mauricio
dc.creatorUribe Lorío, Lidieth
dc.creatorWingChing Jones, Rodolfo
dc.creatorUsaga Barrientos, Jessie
dc.creatorBarboza Vargas, Natalia María
dc.date.accessioned2021-11-17T14:22:06Z
dc.date.available2021-11-17T14:22:06Z
dc.date.issued2021
dc.identifier.urihttps://hdl.handle.net/10669/85214
dc.description.abstractBackground. Agro-industrial waste from tropical environments could be an important source of lactic acid bacteria (LAB) with probiotic potential. Methods. Twelve LAB isolates were isolated from pineapple silages. The species identification was carried out considering 16S rRNA and pheS genes. Experiments to evaluate the probiotic potential of the isolates included survival under simulated gastrointestinal environment, in vitro antagonistic activity (against Salmonella spp. and Listeria monocytogenes), auto-aggregation assays, antibiotic susceptibility, presence of plasmids, adhesiveness to epithelial cells, and antagonistic activity against Salmonella in HeLa cells. Results. Lacticaseibacillus paracasei, Lentilactobacillus parafarraginis, Limosilactobacillus fermentum, and Weissella ghanensis were identified. Survival of one of the isolates was 90% or higher after exposure to acidic conditions (pH: 2), six isolates showed at least 61% survival after exposure to bile salts. The three most promising isolates, based on survivability tests, showed a strong antagonistic effect against Salmonella. However, only L. paracasei_6714 showed a strong Listeria inhibition pattern; this isolate showed a good auto-aggregation ability, was resistant to some of the tested antibiotics but was not found to harbor plasmids; it also showed a high capacity for adhesion to epithelial cells and prevented the invasion of Salmonella in HeLa cells. After further in vivo evaluations, L. paracasei_6714 may be considered a probiotic candidate for food industry applications and may have promising performance in acidic products due to its origines_ES
dc.description.sponsorshipFondo de Incentivos MICITT/CONICIT/[FI-031B-14]//Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourcePeerJ, pp.1-51es_ES
dc.subjectPineapple silageses_ES
dc.subjectLactic acid bacteriaes_ES
dc.subjectProbiotic potentiales_ES
dc.titleFirst characterization of the probiotic potential of lactic acid bacteria isolated from Costa Rican pineapple silageses_ES
dc.typepreprint
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro Nacional de Ciencia y Tecnología de Alimentos (CITA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro de Investigaciones Agronómicas (CIA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.identifier.codproyecto735-B9-457
dc.identifier.codproyecto735-B8-610


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