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dc.creatorSimões, Letícia O.
dc.creatorAlves, Quiara L.
dc.creatorCamargo, Samuel B.
dc.creatorAraújo, Fênix A.
dc.creatorHora, Viviane R. S.
dc.creatorJesus, Rafael L. C.
dc.creatorBarreto, Breno C.
dc.creatorMacambira, Simone G.
dc.creatorSoares, Milena B. P.
dc.creatorMeira, Cássio S.
dc.creatorAguiar, Márcio C.
dc.creatorCouto, Ricardo D.
dc.creatorLomonte, Bruno
dc.creatorMenezes Filho, José Evaldo
dc.creatorCruz, Jader S.
dc.creatorVannier Santos, Marcos A.
dc.creatorCasais e Silva, Luciana L.
dc.creatorSilva, Darizy F.
dc.date.accessioned2021-04-22T15:07:42Z
dc.date.available2021-04-22T15:07:42Z
dc.date.issued2021
dc.identifier.citationhttps://www.sciencedirect.com/science/article/abs/pii/S0378427420304823?via%3Dihubes_ES
dc.identifier.urihttps://hdl.handle.net/10669/83288
dc.description.abstractEnvenoming, resulting from snake bites, is a global public health problem. The present study was undertaken to investigate the influence of Crotalus durissus cascavella (Cdcas) venom on cardiac activity and the mechanisms of action underlying its effect. To investigate the inotropic and chronotropic effects induced by Cdcas, studies were performed on the left and right atria. A series of tests were conducted to investigate whether the negative inotropic effect, induced by Cdcas, was related to cardiac damage. Cdcas venom (0.1−30 μg/mL) elicited a significant negative inotropic effect. The addition of Cdcas crude venom (7.5, 15 and 30 μg/mL) did not induce significant alterations in cell proliferation, nor in the enzymatic activity of total-CK and CKsingle bondMB. Ultrastructural evaluation demonstrated that cardiac cells from isoproterenol and Cdcas groups revealed discreet swelling and displaced intermyofibrillar mitochondria with disorganization of the cristae. No change was observed in cardiac electrical activity in perfused isolated rat hearts with Cdcas. In addition, Cdcas reduced contractility in isolated cardiomyocytes from the rat left ventricle. The negative inotropic effect of Cdcas was reduced by l-NAME (100 μM), PTIO (100 μM), ODQ (10 μM) and KT5823 (1 μM), suggesting the participation of NO/cGMP/PKG pathway due to Cdcas. In non-anesthetized rats, Cdcas induced hypotension followed by bradycardia, the latter was also observed by ECG (anesthetized animals). Our results suggest that the negative inotropic effect induced by Cdcas venom is unrelated to cardiac toxicity, at least, at the concentrations tested; and occurs through of NO/cGMP/PKG pathway, likely leading to hypotension and bradycardia when administered in vivo.es_ES
dc.language.isoenges_ES
dc.sourceToxicology Letters (2021) 337, 121-133es_ES
dc.subjectcardiotoxicityes_ES
dc.subjectCrotaluses_ES
dc.subjectsnake venomes_ES
dc.titleCardiac effect induced by Crotalus durissus cascavella venom: morphofunctional evidence and mechanism of actiones_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.toxlet.2020.11.019
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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