Effect of exposition to chlorpyrifos upon plasmatic cholinesterases, hematology and blood biochemistry values in Bothrops asper (Serpentes: Viperidae)
artículo original
Fecha
2018Autor
Arguedas Porras, Randall
Gómez Argüello, Aarón
Barquero Arroyo, Marco David
Chacón Gutiérrez, Danilo
Corrales Chaves, Greivin
Hernández, Sonia M.
León Montero, Guillermo
Metadatos
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The terciopelo (Bothrops asper), is one of the most important venomous snakes in Costa Rica and common on agriculture where insecticides are frequently used for pest control. To assess the exposure to
organophosphates on captive B. asper, an experiment using chlorpyrifos and butyrylcholinesterase (BChE), as a biomarker was conducted. In addition to BChE, hematology, aspartate aminotransferase
(AST), total proteins (TP) and albumin were measured after exposure. Different concentrations of
chlorpyrifos were used in Group A (0.1%) and B (1%), while the Control Group received distilled water;
each group was composed of 5 snakes. Values of BChE, AST, TP, and albumin were measured before
exposure, and at 6, 12, 24, 196, 360 and 528 h post-exposure. Hematology values were measured after
24 h post-exposure. As result, an important variation between subjects in all groups before exposure was
obtained. Moreover, BChE activity showed 37% inhibition of Group A when compared to Control Group at
12 h post-exposure, and a higher inhibition of Group B (97%) related to Control Group, at 6 h postexposure. Recovery of BChE occurred towards 528 h, never reaching initial values. Despite some variation in the rest of parameters used, a marked relative lymphopenia and monocytosis occurred at 24 h, assuming stress as the main cause.
External link to the item
10.1016/j.chemosphere.2018.04.111Colecciones
- Biología [1635]