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dc.creatorAlvarado Gámez, Ana Lorena
dc.creatorAlonso Lomillo, María Asunción
dc.creatorDomínguez Renedo, Olga
dc.creatorArcos Martínez, María Julia
dc.date.accessioned2020-02-28T15:48:24Z
dc.date.available2020-02-28T15:48:24Z
dc.date.issued2015
dc.identifier.citationhttps://www.mdpi.com/1424-8220/15/2/2232es_ES
dc.identifier.issn1424-8220
dc.identifier.urihttps://hdl.handle.net/10669/80662
dc.description.abstractThis paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-l-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 μM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 μM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[804-B2-297]/UCR/Costa Ricaes_ES
dc.description.sponsorshipJunta de Castilla y León/[BU212A12-2]//Españaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[TEC2009-12029]/MICINN/Españaes_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional/[INNPACTO SERIBIO 2011-2014]/FEDER/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceSensors, vol.15(2), pp.2232-2243es_ES
dc.subjectAlkaline phosphatasees_ES
dc.subjectChronoamperometryes_ES
dc.subject2-phospho-l-ascorbic acid trisodium saltes_ES
dc.subjectScreen printed carbon electrodees_ES
dc.subjectTungstenes_ES
dc.titleA Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-l-Ascorbic Acid Trisodium Salt as a Substratees_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.3390/s150202232
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.identifier.codproyecto804-B2-297
dc.identifier.pmidPMC4367303
dc.identifier.pmid25621602


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