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dc.creatorCambronero Heinrichs, Juan Carlos
dc.creatorMatarrita Carranza, Bernal
dc.creatorMurillo Cruz, Catalina
dc.creatorAraya Valverde, Emanuel
dc.creatorChavarría Vargas, Max
dc.creatorPinto Tomás, Adrián A.
dc.date.accessioned2019-11-26T17:08:12Z
dc.date.available2019-11-26T17:08:12Z
dc.date.issued2019
dc.identifier.citationhttps://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000754es_ES
dc.identifier.govdocVI-3305-2014
dc.identifier.issn1350-0872
dc.identifier.urihttps://hdl.handle.net/10669/79932
dc.description.abstractMany insects have been associated with actinobacteria in protective symbiosis where antimicrobial metabolites inhibit host pathogens. However, the microbiota of neotropical insects such as the stingless-bee Tetragonisca angustula is poorly explored. T. angustula is a meliponid bee widely distributed in Latin America, its honey is traditionally exploited because of its ethno-pharmacological properties and its antimicrobial activity has been demonstrated. Also, the well-structured nest of this species allows exploration of the microbiota of its different components. Even though Streptomyces spp. have been cultured from stingless-bees, little is known about their role in this insect–microbe relationship. In this study, we examined the association between culturable actinobacteria and T. angustula, and evaluated the isolates’ potential as antimicrobial producers. We isolated 51 actinobacteria from adult bees and different substrates of the hive of T. angustula (pollen and honey storage, garbage pellets and cerumen). We then performed a 16S rRNA phylogenetic analysis that clusters the bacteria to previously described lineages of host-associated Streptomyces. In addition, all the isolates were classified according to their antibacterial activity against human pathogens, measured by a growth inhibition test based on diffusion in agar. More than 50 % of our isolates exhibit antimicrobial activity, mainly to Gram-positive bacteria and fungi and only two against Gram-negative bacteria. Additionally, we obtained electron micrographs of adult bees with what appears to be patches of hyphae with Streptomyces-like cell morphology on their body surface. Our results suggest that T. angustula possibly uptakes and transfers actinobacteria from the environment, acting as vectors for these potentially beneficial organisms. This research provides new insights regarding the microbiota associated with T. angustula and justify future studies exploring the full diversity of the microbial community associated with the hive and the possible exchange of microbes with the crops they pollinate.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[810-B3-185]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceMicrobiology, vol. 165(3), pp.292-301es_ES
dc.subjectTetragonisca angustulaes_ES
dc.subjectAntimicrobial-producing Streptomyceses_ES
dc.subjectStingless-beees_ES
dc.subjectActinobacteria vectoringes_ES
dc.titlePhylogenetic analyses of antibiotic-producing Streptomyces sp. isolates obtained from the stingless-bee Tetragonisca angustula (Apidae: Meliponini)es_ES
dc.typeartículo científicoes_ES
dc.date.updated2019-11-19T20:48:33Z
dc.identifier.doi10.1099/mic.0.000754
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Estructuras Microscópicas (CIEMIC)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro en Investigación en Contaminación Ambiental (CICA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.identifier.codproyecto810-B3-185


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