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Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts
dc.creator | Chacón Díaz, Carlos | |
dc.creator | Altamirano Silva, Pamela | |
dc.creator | González Espinoza, Gabriela | |
dc.creator | Medina, María Concepción | |
dc.creator | Alfaro Alarcón, Alejandro | |
dc.creator | Bouza Mora, Laura | |
dc.creator | Jiménez Rojas, César | |
dc.creator | Wong Araya, Melissa | |
dc.creator | Barquero Calvo, Elías | |
dc.creator | Rojas Campos, Norman | |
dc.creator | Guzmán Verri, Caterina | |
dc.creator | Moreno Robles, Edgardo | |
dc.creator | Chaves Olarte, Esteban | |
dc.date.accessioned | 2019-04-08T16:48:46Z | |
dc.date.available | 2019-04-08T16:48:46Z | |
dc.date.issued | 2015-11-10 | |
dc.identifier.citation | https://iai.asm.org/content/83/12/4861 | |
dc.identifier.issn | 1098-5522 | |
dc.identifier.uri | https://hdl.handle.net/10669/76879 | |
dc.description.abstract | Canine brucellosis caused by Brucella canis is a disease of dogs and a zoonotic risk. B. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium is an intracellular pathogen. Studies of B. canis outbreaks in kennel facilities indicated that infected dogs displaying clinical signs did not present hematological alterations. A virulent B. canis strain isolated from those outbreaks readily replicated in different organs of mice for a protracted period. However, the levels of tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-12 in serum were close to background levels. Furthermore, B. canis induced lower levels of gamma interferon, less inflammation of the spleen, and a reduced number of granulomas in the liver in mice than did B. abortus. When the interaction of B. canis with cells was studied ex vivo, two patterns were observed, a predominant scattered cell-associated pattern of nonviable bacteria and an infrequent intracellular replicative pattern of viable bacteria in a perinuclear location. The second pattern, responsible for the increase in intracellular multiplication, was dependent on the type IV secretion system VirB and was seen only if the inoculum used for cell infections was in early exponential phase. Intracellular replicative B. canis followed an intracellular trafficking route undistinguishable from that of B. abortus. Although B. canis induces a lower proinflammatory response and has a stealthier replication cycle, it still displays the pathogenic properties of the genus and the ability to persist in infected organs based on the ability to multiply intracellularly. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[803-B3-761]UCR/Costa Rica | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[803-B4-654]UCR/Costa Rica | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[803-B5-653]UCR/Costa Rica | es_ES |
dc.description.sponsorship | Consejo Nacional de Ciencia y Tecnología de Costa Rica/[FV-0004-13]/CONICIT/Costa Rica | es_ES |
dc.description.sponsorship | International Center for Genomic Engineering and Biotechnology/[CRP/12/007]/ICGEB/India | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Infection and Immunity, vol. 83(12), pp. 4861–4870 | es_ES |
dc.subject | Anine brucellosis | es_ES |
dc.subject | Dogs | es_ES |
dc.subject | 579.33 Bastoncitos y cocos aeróbicos gram-negativos | es_ES |
dc.title | Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1128/IAI.00995-15 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.codproyecto | 803-B4-654 | |
dc.identifier.codproyecto | 803-B5-653 | |
dc.identifier.codproyecto | 803-B3-761 |
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