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dc.creatorQuesada Gómez, Carlos
dc.creatorRodríguez Cavallini, Evelyn
dc.creatorRodríguez Sánchez, César
dc.date.accessioned2018-07-04T17:53:17Z
dc.date.available2018-07-04T17:53:17Z
dc.date.issued2013-06
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S1075996413000334?via%3Dihubes_ES
dc.identifier.issn1075-9964
dc.identifier.urihttps://hdl.handle.net/10669/75157
dc.description.abstractThe frequency of finding of clindamycin-resistant anaerobic bacteria in clinical samples has doubled from 2008 to 2010 in Costa Rica. To determine whether this increase is due to dissemination of erm genes aided by tetQ elements, we analyzed 100 isolates of Bacteroides or Parabacteroides from a regional hospital, a national hospital, and the community. Antimicrobial susceptibilities were recorded with a broth micro-dilution method and erm genes were detected by PCR and Southern blotting. In addition, plasmid isolation and mating experiments were performed to clarify the location and mobility of the detected erm genes. Resistance to clindamycin was by far more frequent in the regional hospital (72%) than in the national hospital (29%) and the community (26%). Resistance to tetracycline was even more common, with the community (85%) outweighing the hospitals (71–72%). While MIC of clindamycin were higher in the hospitals than in the community (P < 0.05), the opposite was seen for tetracycline (P < 0.0001). Of the sought-after genes, only ermG (n = 2), ermA (n = 1), and ermF (n = 1) were detected in the hospitals and ermF in the community (n = 2). In opposition to the low frequency of finding of erm genes, 71% of the isolates were positive for tetQ. None of the detected genes were encoded on plasmids. Only three isolates from the hospitals transferred their erm genes laterally. By contrast, 13 hospital isolates and two community isolates transferred tetQ. Despite the widespread finding of tetracycline-resistant tetQ-positive bacteria, mobile erm genes were rare in our bacterial collection. We conclude that the detected erm genes are likely not included in typical conjugative transposons of Bacteroides and Parabacteroides.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[ED-2039]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B1-142]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourceAnaerobe, Vol 21, pp 18-21es_ES
dc.subjectBacteroideses_ES
dc.subjectParabacteroideses_ES
dc.subjectClindamycines_ES
dc.subjectTetracyclinees_ES
dc.subjectErm geneses_ES
dc.subjecttetQes_ES
dc.subjectConjugationes_ES
dc.subject579.314 972 86 Bacterias anaeróbicases_ES
dc.titleScarce detection of mobile erm genes associated with tetQ in Bacteroides and Parabacteroides from Costa Ricaes_ES
dc.typeartículo científicoes_ES
dc.date.updated2018-06-06T14:43:40Z
dc.identifier.doi10.1016/j.anaerobe.2013.03.006
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.codproyecto803-B1-142
dc.identifier.codproyectoED-2039


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