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dc.creatorLewin, Gina R.
dc.creatorJohnson, Amanda L.
dc.creatorMoreira Soto, Rolando Daniel
dc.creatorPerry, Kailene
dc.creatorBook, Adam J.
dc.creatorHorn, Heidi A.
dc.creatorPinto Tomás, Adrián A.
dc.creatorCurrie, Cameron Robert
dc.description.abstractDeconstruction of the cellulose in plant cell walls is critical for carbon flow through ecosystems and for the production of sustainable cellulosic biofuels. Our understanding of cellulose deconstruction is largely limited to the study of microbes in isolation, but in nature, this process is driven by microbes within complex communities. In Neotropical forests, microbes in leaf-cutter ant refuse dumps are important for carbon turnover. These dumps consist of decaying plant material and a diverse bacterial community, as shown here by electron microscopy. To study the portion of the community capable of cellulose degradation, we performed enrichments on cellulose using material from five Atta colombica refuse dumps. The ability of enriched communities to degrade cellulose varied significantly across refuse dumps. 16S rRNA gene amplicon sequencing of enriched samples identified that the community structure correlated with refuse dump and with degradation ability. Overall, samples were dominated by Bacteroidetes, Gammaproteobacteria, and Betaproteobacteria. Half of abundant operational taxonomic units (OTUs) across samples were classified within genera containing known cellulose degraders, including Acidovorax, the most abundant OTU detected across samples, which was positively correlated with cellulolytic ability. A representative Acidovorax strain was isolated, but did not grow on cellulose alone. Phenotypic and compositional analyses of enrichment cultures, such as those presented here, help link community composition with cellulolytic ability and provide insight into the complexity of community-based cellulose degradation.es_ES
dc.description.sponsorshipBiological and Environmental Research/[DE-FC02-07ER64494]/BER/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[DGE-1256259]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[DEB-0747002]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[MCB-0702025]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Institutes of Health/[T32 GM07215]/NIH/Estados Unidoses_ES
dc.description.sponsorshipUniversidad de Costa Rica/[]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia, Tecnología y Telecomunicaciones/[]/MICITT/Costa Ricaes_ES
dc.description.sponsorshipUniversity of Wisconsin-Madison's Hilldale Undergraduate Faculty Research Fellowship/[]//Estados Unidoses_ES
dc.rightsAtribución 3.0 Costa Rica*
dc.sourcePLOS ONE; Volumen 11, Número 3, 2016es_ES
dc.subjectAnt (Atta colombica)es_ES
dc.subjectFilter paperes_ES
dc.subjectRibosomal RNAes_ES
dc.subjectSequence databaseses_ES
dc.titleCellulose-Enriched Microbial Communities from Leaf-Cutter Ant (Atta colombica) Refuse Dumps Vary in Taxonomic Composition and Degradation Abilityes_ES
dc.typeartículo científicoes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES

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Atribución 3.0 Costa Rica
Except where otherwise noted, this item's license is described as Atribución 3.0 Costa Rica