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dc.creatorCaccin, Paola
dc.creatorPellegatti, Patrizia
dc.creatorFernández Ulate, Julián
dc.creatorVono, Maria
dc.creatorCintra Francischinelli, Mariana
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorDi Virgilio, Francesco
dc.creatorMontecucco, Cesare
dc.date.accessioned2017-06-09T17:12:30Z
dc.date.available2017-06-09T17:12:30Z
dc.date.issued2013-01-25
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0006291X12023893es_ES
dc.identifier.issn0006-291X
dc.identifier.urihttps://hdl.handle.net/10669/30081
dc.description.abstractThe venom of the snake Bothrops asper causes muscle necrosis, pain and inflammation. This venom contains myotoxins which cause an increase in intracellular Ca2+ concentration and release of K+ and ATP from myotubes. ATP is a key danger molecule that triggers a variety of reactions, including activation of the innate immune response. Here, using ATP-luciferase bioluminescence imaging technique, we show for the first time in vivo, that the purified myotoxins induce rapid release of ATP, whilst the complete venom of B. asper does at a very small extent. This apparent contradiction is explained by the finding that the venom contains powerful nucleotidases that in vivo convert ATP into ADP, AMP and Adenosine. These findings indicate that high concentrations of adenosine are generated by the double action of the venom and provide the experimental basis to the suggestion that in situ generated adenosine plays an important role in envenomation via its hypotensive, paralyzing and anti-coagulant activities.es_ES
dc.description.sponsorshipUniversità di Padova Progetto Strategico///Italiaes_ES
dc.description.sponsorshipFondazione CARIPARO/[ICGEB-CRP Program (grant COS-08-03]//Italiaes_ES
dc.description.sponsorshipItalian Association for Cancer Research/[IG n. 5354]//Italiaes_ES
dc.description.sponsorshipTelethon/[GGP06070]//Italiaes_ES
dc.description.sponsorshipCommission of European Communities (ERA-NET ‘‘Nanostroke’’)///es_ES
dc.description.sponsorshipRegione Emilia Romagna (Research Programs ‘‘Innovative approaches to the diagnosis of inflammatory diseases’’ and ‘‘Moniter’’)///es_ES
dc.description.sponsorshipUniversity of Ferrara///Italiaes_ES
dc.language.isoen_USes_ES
dc.sourceBiochemical and Biophysical Research Communications; Volumen 430, Número 4. 2013es_ES
dc.subjectBothropses_ES
dc.subjectATPes_ES
dc.subjectChemiluminescencees_ES
dc.subjectAdenosinees_ES
dc.subjectNucleotidaseses_ES
dc.subjectSnake venomes_ES
dc.titleWhy myotoxin-containing snake venoms possess powerful nucleotidases?es_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.bbrc.2012.11.129
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid23261426


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