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dc.creatorZamunér, Stella Regina
dc.creatorGutiérrez, José María
dc.creatorMuscará, Marcelo N.
dc.creatorTeixeira, Simone A.
dc.creatorTeixeira, Catarina de Fátima
dc.date.accessioned2017-02-02T15:31:01Z
dc.date.available2017-02-02T15:31:01Z
dc.date.issued2001-10
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010101001234es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/29481
dc.description.abstractVenoms from snakes of the genus Bothrops cause pronounced local effects in the victims. These alterations result not only from the direct toxic action of venom components, but also from the prominent inflammatory reaction associated with these envenomations. In this study we investigated the ability of Bothrops asper (BaV) and Bothrops jararaca (BjV) venoms to induce cellular influx and microbicidal functions in leukocytes. BaV and BjV (5 μg/animal) caused a long lasting infiltration of leukocytes (3–48 h) when injected into mouse peritoneal cavity. Both venoms increased phagocytosis and production of hydrogen peroxide (H2O2) by polymorphonuclear (PMN) and mononuclear (MN) peritoneal leukocytes. In addition, nitric oxide (NO) production by macrophages was also enhanced after the venom injections. This effect was inhibited by treating animals with L-NAME and aminoguanidine, thus suggesting the induction of iNOS synthesis by the venoms. Western blot analysis confirmed the expression of iNOS in macrophages. BaV and BjV injection led to increased levels of IFN-γ at the site of inflammation. Since IFN-γ is an effective inducer of iNOS expression, an indirect action of the venoms on iNOS expression can be proposed. A marked formation of nitrotyrosine-containing proteins was also observed in macrophage homogenates. Based on these results, we suggest that reactive oxygen and nitrogen-derived species are involved in the pathogenesis of the local tissue damage characteristic of Bothrops sp envenomations.es_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[98/00162-9]/FAPESP/Brasiles_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[97/13089-5]/FAPESP/Brasiles_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[95/9699-7]/FAPESP/Brasiles_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico/[301199/91-4)]/CNPq/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 39, Número 10, 2001es_ES
dc.subjectHydrogen Peroxidees_ES
dc.subjectNitric Oxidees_ES
dc.subjectPeroxynitritees_ES
dc.subjectLeukocyte Functionses_ES
dc.subjectSnake venomes_ES
dc.titleBothrops asper and Bothrops jararaca snake venoms trigger microbicidal functions of peritoneal leukocytes in vivoes_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/S0041-0101(01)00123-4
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid11478958


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