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Venom of the crotaline snake Atropoides nummifer (jumping viper) from Guatemala and Honduras: comparative toxicological characterization, isolation of a myotoxic phospholipase A(2) homologue and neutralization by two antivenoms
dc.creator | Rojas Umaña, Ermila | |
dc.creator | Saravia Otten, Patricia | |
dc.creator | Angulo Ugalde, Yamileth | |
dc.creator | Arce, Viviana | |
dc.creator | Lomonte, Bruno | |
dc.creator | Chávez, Juan José | |
dc.creator | Velásquez, Rubén | |
dc.creator | Thelestam, Mónica | |
dc.creator | Gutiérrez, José María | |
dc.date.accessioned | 2017-02-01T21:37:12Z | |
dc.date.available | 2017-02-01T21:37:12Z | |
dc.date.issued | 2001-07 | |
dc.identifier.issn | 1532-0456 | |
dc.identifier.uri | https://hdl.handle.net/10669/29478 | |
dc.description.abstract | A comparative study was performed on the venoms of the crotaline snake Atropoides nummifer from Guatemala and Honduras. SDS-polyacrylamide gel electrophoresis, under reducing conditions, revealed a highly similar pattern of these venoms, and between them and the venom of the same species from Costa Rica. Similar patterns were also observed in ion-exchange chromatography on CM-Shephadex C-25, in which a highly basic myotoxic fraction was present. This fraction was devoid of phospholipase A(2) activity and strongly reacted, by enzyme-immunoassay, with an antiserum against Bothrops asper myotoxin II, a Lys-49 phospholipase A(2) homologue. A basic myotoxin of 16 kDa was isolated to homogeneity from the venom of A. nummifer from Honduras, showing amino acid composition and N-terminal sequence similar to those of Lys-49 phospholipase A(2) variants previously isolated from other crotaline snake venoms. Guatemalan and Honduran A. nummifer venoms have a qualitatively similar toxicological profile, characterized by: lethal; hemorrhagic; myotoxic; edema-forming; coagulant; and defibrinating activities, although there were significant quantitative variations in some of these activities between the two venoms. Neutralization of toxic activities by two commercially-available antivenoms in the region was studied. Polyvalent antivenom produced by Instituto Clodomiro Picado was effective in the neutralization of: lethal; hemorrhagic; myotoxic; coagulant; defibrinating; and phospholipase A(2) activities, but ineffective against edema-forming activity. On the other hand, MYN polyvalent antivenom neutralized: hemorrhagic; myotoxic; coagulant; defibrinating; and phospholipase A(2) activities, albeit with a lower potency than Instituto Clodomiro Picado antivenom. MYN antivenom failed to neutralize lethal and edema-forming activities of A. nummifer venoms. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[741-99-269]/UCR/Costa Rica | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[741-89-057]/UCR/Costa Rica | es_ES |
dc.description.sponsorship | Network for Research and Training in Tropical Diseases in Central America//NeTropica/ | es_ES |
dc.description.sponsorship | International Foundation for Science/[2677-1]/IFS/Suecia | es_ES |
dc.description.sponsorship | Consejo Nacional de Ciencia y Tecnología/[MS-058-1]/CONICYT/Guatemala | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Comparative Biochemistry and Physiology Part C: Toxicology & pharmacology; Volumen 129, Número 2. 2001 | es_ES |
dc.subject | Amino Acid Sequence | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Antivenins | es_ES |
dc.subject | Cells, Cultured | es_ES |
dc.subject | Chromatography, High Pressure Liquid | es_ES |
dc.subject | Chromatography, Ion Exchange | es_ES |
dc.subject | Coagulants | es_ES |
dc.subject | Cross Reactions | es_ES |
dc.subject | Crotalid Venoms | es_ES |
dc.subject | Edema | es_ES |
dc.subject | Electrophoresis, Polyacrylamide Gel | es_ES |
dc.subject | Fibrinolysis | es_ES |
dc.subject | Guatemala | es_ES |
dc.subject | Hemorrhage | es_ES |
dc.subject | Honduras | es_ES |
dc.subject | In Vitro Techniques | es_ES |
dc.subject | Injections, Intradermal | es_ES |
dc.subject | Injections, Intramuscular | es_ES |
dc.subject | Injections, Intraperitoneal | es_ES |
dc.subject | Lethal Dose 50 | es_ES |
dc.subject | Mice | es_ES |
dc.subject | Muscle, Skeletal | es_ES |
dc.subject | Neutralization Tests | es_ES |
dc.subject | Phospholipases A | es_ES |
dc.subject | Viperidae | es_ES |
dc.subject | Snake venom | es_ES |
dc.title | Venom of the crotaline snake Atropoides nummifer (jumping viper) from Guatemala and Honduras: comparative toxicological characterization, isolation of a myotoxic phospholipase A(2) homologue and neutralization by two antivenoms | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/s1532-0456(01)00198-3 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.codproyecto | 741-89-057 | |
dc.identifier.codproyecto | 741-99-269 | |
dc.identifier.pmid | 11423387 | |
dc.identifier.pmid | 11423387 |
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