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Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper
dc.creator | Pérez, Aida Verónica | |
dc.creator | Rucavado Romero, Alexandra | |
dc.creator | Sanz, Libia | |
dc.creator | Calvete Chornet, Juan José | |
dc.creator | Gutiérrez, José María | |
dc.date.accessioned | 2016-11-29T20:57:10Z | |
dc.date.available | 2016-11-29T20:57:10Z | |
dc.date.issued | 2008-01 | |
dc.identifier.citation | http://www.bjournal.com.br/index.php?option=com_jresearch&view=publication&task=show&id=10377 | |
dc.identifier.issn | 1414-431X | |
dc.identifier.uri | https://hdl.handle.net/10669/29337 | |
dc.description.abstract | A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose and ion-exchange chromatography on DEAE-Sepharose. The enzyme accounts for approximately 0.13% of the venom dry weight and has a molecular mass of 32 kDa as determined by SDS-PAGE, and of 27 kDa as determined by MALDI-TOF mass spectrometry. Its partial amino acid sequence shows high identity with snake venom serine proteinases and a complete identity with a cDNA clone previously sequenced from this species. The N-terminal sequence of the enzyme is VIGGDECNINEHRSLVVLFXSSGFL CAGTLVQDEWVLTAANCDSKNFQ. The enzyme induces clotting of plasma (minimum coagulant dose = 4.1 µg) and fibrinogen (minimum coagulant dose = 4.2 µg) in vitro, and promotes defibrin(ogen)ation in vivo (minimum defibrin(ogen)ating dose = 1.0 µg). In addition, when injected intravenously in mice at doses of 5 and 10 µg, it induces a series of behavioral changes, i.e., loss of the righting reflex, opisthotonus, and intermittent rotations over the long axis of the body, which closely resemble the `gyroxin-like' effect induced by other thrombin-like enzymes from snake venoms.. | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Brazilian Journal of Medical and Biological Research; Volumen 41, Número 1. 2008 | es_ES |
dc.subject | Bothrops Asper | es_ES |
dc.subject | Serine Proteinase | es_ES |
dc.subject | Thrombin-like serine proteinase | es_ES |
dc.subject | Defibrin(ogen)ation | es_ES |
dc.subject | Snake venom | es_ES |
dc.title | Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper | es_ES |
dc.type | artículo original | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
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Microbiología [1171]