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dc.creatorCalderón Fuentes, Leoneles_ES
dc.creatorLomonte, Brunoes_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.creatorTarkowski, Andrejes_ES
dc.creatorHanson, Lars Akees_ES
dc.date.accessioned2016-11-11T14:11:52Zes_ES
dc.date.available2016-11-11T14:11:52Zes_ES
dc.date.issued1993-06es_ES
dc.identifier.issn0041-0101es_ES
dc.identifier.urihttps://hdl.handle.net/10669/29224es_ES
dc.description.abstractVipera berus is widely distributed throughout the northern part of Europe and Asia. Characterization of several toxic effects of its venom in the mouse, as well as of in vitro enzymatic activities was performed. Vipera berus venom displayed in vitro proteolytic, fibrinolytic, anticoagulant, and phospholipase A2 activities. The i.p. LD50 of the venom for Swiss mice was 0.86 micrograms/g (95% confidence limits 0.71-1.01 microgram/g). Significant local tissue-damaging effects, including edema, hemorrhage and myonecrosis, were observed. The local edema was characterized by rapid onset, reaching a maximum after 0.5-1 hr, and with dose-dependent persistence. The hemorrhagic potency was measured by a skin test, giving a minimum hemorrhagic dose value of 3.2 micrograms. The venom also induced a moderate local myonecrosis, evidenced by histological evaluation of injected tissue (gastrocnemius), and by biochemical parameters (increase of plasma creatine kinase activity, and decrease of muscle residual MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide)-reducing activity). Characterization of the venom by SDS-polyacrylamide gel electrophoresis revealed 10 (reduced) or 11 (unreduced) main protein bands, which were further analyzed in relation to mol. wt and relative concentration by densitometry. A rabbit antiserum to V. berus venom recognized all main venom bands by immunoblotting. This antiserum cross-reacted to a variable extent with several crotaline venoms, as assessed by enzyme immunoassay.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 31, Número 6, 1993es_ES
dc.subjectAnimalses_ES
dc.subjectAntibodieses_ES
dc.subjectAntigenses_ES
dc.subjectCross Reactionses_ES
dc.subjectCrotalid Venomses_ES
dc.subjectDisease Models, Animales_ES
dc.subjectDose-Response Relationship, Druges_ES
dc.subjectEdemaes_ES
dc.subjectElectrophoresis, Polyacrylamide Geles_ES
dc.subjectImmunoblottinges_ES
dc.subjectLethal Dose 50es_ES
dc.subjectMicees_ES
dc.subjectPeptide Hydrolaseses_ES
dc.subjectRabbitses_ES
dc.subjectSodium Dodecyl Sulfatees_ES
dc.subjectViper Venomses_ES
dc.subjectSnake venomes_ES
dc.titleBiological and biochemical activities of Vipera berus (European viper) venomes_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/0041-0101(93)90380-2es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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