dc.creator | Rojas Céspedes, Gustavo | |
dc.creator | Vargas, Marianela A. | |
dc.creator | Robles, Abel | |
dc.creator | Gutiérrez, José María | |
dc.date.accessioned | 2016-11-11T13:52:00Z | |
dc.date.available | 2016-11-11T13:52:00Z | |
dc.date.issued | 1993 | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/29221 | |
dc.description.abstract | Twenty batches of polyvalent antivenom produced at the Instituto Clodomiro Picado were analyzed for turbidity, both before and after freezing-thawing and lyophilization. Eight batches became turbid upon freezing-thawing, and this change correlated with high levels of cholesterol, triglycerides and lipoproteins, especially beta-lipoprotein. Since normal horse serum does not become turbid after freezing-thawing, despite the fact that it has high lipoprotein levels, the possibility was raised that phenol, used as a preservative during serum fractionation, might affect lipoproteins, inducing the appearance of turbidity after freezing-thawing. This hypothesis was tested by fractionating a sample of hyperimmune serum either without phenol or using two different phenol concentrations (0.1 g/dl and 0.25 g/dl). Results showed that, although the three samples had the same cholesterol and triglyceride levels before fractionation, only the one having 0.25 g/dl phenol became turbid upon freezing-thawing, containing a diffuse lipoprotein band on electrophoresis. This finding suggests that turbidity in equine antivenoms depends on the interaction of at least three factors: (a) freezing, (b) high initial cholesterol and lipoprotein concentration in the serum, and (c) addition of phenol during fractionation of serum. | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon 31(1):61-63. 1993 | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Antivenins | es_ES |
dc.subject | Cholesterol | es_ES |
dc.subject | Freezing | es_ES |
dc.subject | Horses | es_ES |
dc.subject | Lipoproteins | es_ES |
dc.subject | Nephelometry and Turbidimetry | es_ES |
dc.subject | Phenol | es_ES |
dc.subject | Triglycerides | es_ES |
dc.subject | Snake venom | es_ES |
dc.title | Turbidity of hyperimmune equine antivenom: the role of phenol and serum lipoproteins | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/0041-0101(93)90357-O | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología | es_ES |