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dc.creatorValiente Álvarez, Carmen Isabel
dc.creatorMoreno Robles, Edgardo
dc.creatorSittenfeld Appel, Ana
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.date.accessioned2016-11-10T15:20:55Z
dc.date.available2016-11-10T15:20:55Z
dc.date.issued1992
dc.identifier.issn0040-0101
dc.identifier.urihttps://hdl.handle.net/10669/29176
dc.description.abstractThe number and isoelectric points of phospholipase A2 isoenzymes were studied in the venoms of 12 Central American crotaline snakes of the genera Bothrops, Crotalus, Lachesis and Agkistrodon. The study was carried out by using a methodology based on electrophoretic separation of venoms, transfer to nitrocellulose and detection of activity of the bands by an indirect hemolytic assay in agarose-erythrocyte-egg yolk gels. All venoms tested had indirect hemolytic activity, although they varied in the number and isoelectric point of their phospholipases A2. Most venoms had predominantly acidic isoenzymes, with the exception of A. bilineatus which had mainly basic isoenzymes and B. schlegelii which had both acidic and basic isoenzymes. Analysis of interindividual variability in B. asper venom demonstrated that two phospholipase A2 isoenzymes are present in some venoms but absent in others. Polyvalent antivenom was effective in neutralizing phospholipase A2 activity of the 12 venoms tested, when venoms and antivenom were incubated in the fluid phase. This work demonstrates a conspicuous interspecific variability in the number and isoelectric points of phospholipases A2 present in Central American crotaline snake venomses_ES
dc.language.isoen_USes_ES
dc.sourceToxicon. Volumen 30, Número 8, 1992es_ES
dc.subjectAnimalses_ES
dc.subjectCosta Ricaes_ES
dc.subjectCrotalid venomes_ES
dc.subjectElectrophoresises_ES
dc.subjectIsoelectric focusinges_ES
dc.subjectIsoenzymeses_ES
dc.subjectPhospholipases A2es_ES
dc.subjectPhospholipases Aes_ES
dc.subjectSnakees_ES
dc.titleAn electrophoretic study on phospholipase A2 isoenzymes in the venoms of Central American crotaline snakeses_ES
dc.typeartículo científico
dc.identifier.doi10.1016/0041-0101(92)90379-Jes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES


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