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Comparison of the effect of Crotalus simus and Crotalus durissus ruruima venoms on the equine antibody response towards Bothrops asper venom: Implications for the production of polyspecific snake antivenoms 

Dos Santos, María Cristina; Arroyo Portilla, Cynthia; Solano Rojas, Sergio; Herrera Vega, María; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo (2011-02)
Antivenoms are preparations of immunoglobulins purified from the plasma of animals immunized with snake venoms. Depending on the number of venoms used during the immunization, antivenoms can be monospecific (if venom from ...

Venom of Bothrops asper from Mexico and Costa Rica: Intraspecific variation and cross-neutralization by antivenoms 

Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Uscanga Reynell, Alfredo; Ponce de León Rosales, Samuel; Jiménez Corona, María Eugenia; Reta Mares, José Francisco; Gutiérrez, José María; León Montero, Guillermo (2012-01)
Bothrops asper is the species that induces the highest incidence of snakebite envenomation in southern Mexico, Central America and parts of northern South America. The intraspecies variability in HPLC profile and ...

Purification of IgG and albumin from human plasma by aqueous two phase system fractionation 

Vargas Arroyo, Mariángela; Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Angulo Ugalde, Yamileth; Gutiérrez, José María; León Montero, Guillermo (2012-08)
The current shortages in human plasma products at global levels justify the development of new, cost effective plasma fractionation methods. We have developed a fractionation process to obtain immunoglobulin G (IgG) and ...

Pathogenic mechanisms underlying adverse reactions induced by intravenous administration of snake antivenoms 

León Montero, Guillermo; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Gutiérrez, José María (2013-12-15)
Snake antivenoms are formulations of immunoglobulins, or immunoglobulin fragments, purified from the plasma of animals immunized with snake venoms. Their therapeutic success lies in their ability to mitigate the progress ...

Snake venomics and antivenomics of Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan: Keys to understand the variable immune response in horses 

Villalta Arrieta, Mauren; Pla Ferrer, Davinia; Yang, Su Lin; Sanz, Libia; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Chen, Pei Yu; Herrera Vega, María; Estrada Umaña, Ricardo; Cheng, Ya Fen; Lee, Cheng Dow; Cerdas Solís, Maykel; Chiang, Jen Ron; Angulo Ugalde, Yamileth; León Montero, Guillermo; Calvete Chornet, Juan José; Gutiérrez, José María (2012-10-22)
The proteomes of the venoms of the snakes Viridovipera stejnegeri and Protobothrops mucrosquamatus from Taiwan were characterized by N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation ...

Snake Venomics of African Spitting Cobras: Toxin Composition and Assessment of Congeneric Cross-Reactivity of the Pan-African EchiTAb-Plus-ICP Antivenom by Antivenomics and Neutralization Approaches 

Petras, Daniel; Sanz, Libia; Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Solano Centeno, Daniela; Vargas Arroyo, Mariángela; León Montero, Guillermo; Warrell, David A.; Theakston, R. David G.; Harrison, Robert A.; Durfa, Nandul; Nasidi, Abdulsalami; Gutiérrez, José María; Calvete Chornet, Juan José (2011)
Venomic analysis of the venoms of Naja nigricollis, N. katiensis, N. nubiae, N. mossambica, and N. pallida revealed similar compositional trends. The high content of cytotoxins and PLA2s may account for the extensive tissue ...
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Preclinical evaluation of caprylic-acid fractionated IgG antivenom for the treatment of taipan (Oxyuranus scutellatus) envneoming in Papua New Guinea 

Vargas Arroyo, Mariángela; Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Estrada Umaña, Ricardo; Cerdas Solís, Maykel; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; León Montero, Guillermo; Gutiérrez, José María; Williams, David J. (PLoS Neglected Tropical Diseases 5 (5) e1144, 2011-05-17)
Background: Snake bite is a common medical emergency in Papua New Guinea (PNG). The taipan, Oxyuranus scutellatus, inflicts a large number of bites that, in the absence of antivenom therapy, result in high mortality. ...

Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity 

Herrera Vega, María; Fernández Ulate, Julián; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; León Montero, Guillermo; Angulo Ugalde, Yamileth; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2012-04-03)
The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of ...

Low pH formulation of whole IgG antivenom: Impact on quality, safety, neutralizing potency and viral inactivation 

Solano, Silvia; Segura Ruiz, Álvaro; León Montero, Guillermo; Gutiérrez, José María; Burnouf, Thierry (2012-03)
Low pH treatment improves the tolerance to intravenous infusion, the stability, and the viral safety of various therapeutic immunoglobulins G preparations, but has never been evaluated for horse plasma-derived antivenoms. ...

Comparative study of the efficacy and safety of two polyvalent, caprylic acid fractionated [IgG and F(ab0)2] antivenoms, in Bothrops asper bites in Colombia 

Otero Patiño, Rafael; Segura Ruiz, Álvaro; Pereañez, Jaime Andrés; Herrera Vega, María; Angulo Ugalde, Yamileth; León Montero, Guillermo; Gutiérrez, José María; Barona, Jacqueline; Estrada, Sebastián; Quintana, Juan Carlos; Vargas Muñoz, Leidy Johana; Gómez, Juan Pablo; Díaz, Abel; Suárez, Ana María; Fernández, Jorge; Ramírez, Patricia; Fabra, Patricia E.; Perea, Monica; Fernández, Diego; Arroyo, Yobana; Betancur, Dalila; Pupo, Lady; Córdoba, Elkin; Ramírez, Eugenio; Arrieta, Ana Berta; Rivero, Alcides; Mosquera, Diana Carolina; Conrado, Nectty Lorena; Ortiz, Rosina (2012-02)
The efficacy and safety of two polyvalent horse-derived antivenoms in Bothrops asper envenomings were tested in a randomized, double-blind, clinical trial performed in Colombia. Both antivenoms were manufactured from the ...
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