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Comparison of the effect of Crotalus simus and Crotalus durissus ruruima venoms on the equine antibody response towards Bothrops asper venom: Implications for the production of polyspecific snake antivenoms 

Dos Santos, María Cristina; Arroyo Portilla, Cynthia; Solano Rojas, Sergio; Herrera Vega, María; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo (2011-02)
Antivenoms are preparations of immunoglobulins purified from the plasma of animals immunized with snake venoms. Depending on the number of venoms used during the immunization, antivenoms can be monospecific (if venom from ...

Immune Response Towards Snake Venoms 

León Montero, Guillermo; Sánchez Céspedes, Laura Vanessa; Hernández, Andrés; Villalta Arrieta, Mauren; Herrera Vega, María; Segura Ruiz, Álvaro; Estrada Umaña, Ricardo; Gutiérrez, José María (2011)
The immune response involves a complex repertoire of innate and adaptive responses to foreign agents in the organism. The present review focuses on the immune response to snake venoms, including those occurring in snakebite ...

Venom of Bothrops asper from Mexico and Costa Rica: Intraspecific variation and cross-neutralization by antivenoms 

Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Uscanga Reynell, Alfredo; Ponce de León Rosales, Samuel; Jiménez Corona, María Eugenia; Reta Mares, José Francisco; Gutiérrez, José María; León Montero, Guillermo (2012-01)
Bothrops asper is the species that induces the highest incidence of snakebite envenomation in southern Mexico, Central America and parts of northern South America. The intraspecies variability in HPLC profile and ...
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Preclinical evaluation of caprylic-acid fractionated IgG antivenom for the treatment of taipan (Oxyuranus scutellatus) envneoming in Papua New Guinea 

Vargas Arroyo, Mariángela; Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Estrada Umaña, Ricardo; Cerdas Solís, Maykel; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; León Montero, Guillermo; Gutiérrez, José María; Williams, David J. (PLoS Neglected Tropical Diseases 5 (5) e1144, 2011-05-17)
Background: Snake bite is a common medical emergency in Papua New Guinea (PNG). The taipan, Oxyuranus scutellatus, inflicts a large number of bites that, in the absence of antivenom therapy, result in high mortality. ...

Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity 

Herrera Vega, María; Fernández Ulate, Julián; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; León Montero, Guillermo; Angulo Ugalde, Yamileth; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2012-04-03)
The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of ...

Comparative study of the efficacy and safety of two polyvalent, caprylic acid fractionated [IgG and F(ab0)2] antivenoms, in Bothrops asper bites in Colombia 

Otero Patiño, Rafael; Segura Ruiz, Álvaro; Pereañez, Jaime Andrés; Herrera Vega, María; Angulo Ugalde, Yamileth; León Montero, Guillermo; Gutiérrez, José María; Barona, Jacqueline; Estrada, Sebastián; Quintana, Juan Carlos; Vargas Muñoz, Leidy Johana; Gómez, Juan Pablo; Díaz, Abel; Suárez, Ana María; Fernández, Jorge; Ramírez, Patricia; Fabra, Patricia E.; Perea, Monica; Fernández, Diego; Arroyo, Yobana; Betancur, Dalila; Pupo, Lady; Córdoba, Elkin; Ramírez, Eugenio; Arrieta, Ana Berta; Rivero, Alcides; Mosquera, Diana Carolina; Conrado, Nectty Lorena; Ortiz, Rosina (2012-02)
The efficacy and safety of two polyvalent horse-derived antivenoms in Bothrops asper envenomings were tested in a randomized, double-blind, clinical trial performed in Colombia. Both antivenoms were manufactured from the ...

Proteomic and toxinological characterization of the venom of the South African Ringhals cobra Hemachatus haemachatus 

Sánchez Brenes, Andrés; Herrera Vega, María; Villalta Arrieta, Mauren; Solano Centeno, Daniela; Segura Ruiz, Álvaro; Lomonte, Bruno; Gutiérrez, José María; León Montero, Guillermo; Vargas Arroyo, Mariángela (2018-06)
The protein composition and toxinological profile of the venom of the African spitting elapid Hemachatus haemachatus (Ringhals) were characterized by bottom-up proteomics and functional in vitro and in vivo assays. Venom ...
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Industrial Production and Quality Control of Snake Antivenoms 

León Montero, Guillermo; Segura Ruiz, Álvaro; Gómez Argüello, Aarón; Hernández Bolaños, Andrés; Navarro Arias, Diego; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Herrera Vega, María; Gutiérrez, José María (2014)
The production of snake antivenoms involves stages such as production of venom, immunization of animals to generate hyperimmune plasma, immunoglobulin purification, viral inactivation (or removal), and stabilization of the ...

Preclinical assessment of the neutralizing capacity of antivenoms produced in six Latin American countries against medically-relevant Bothrops snake venoms 

Segura Ruiz, Álvaro; Castillo, M. C.; Núñez Rangel, Vitelbina; Yarlequé, A.; Gonçalves, Luís Roberto de Camargo; Villalta Arrieta, Mauren; Bonilla, César; Herrera Vega, María; Fernández, M.; Yano, M. Y.; Araújo, H. P.; Boller, M. A. A.; León, P.; Sano Martins, I. S.; Gómez, A.; Fernández, Gil Patrick; Geoghegan, Patricia; Higashi, Hisako Gondo; León Montero, Guillermo; Gutiérrez, José María; Tintaya, Benigno (2010-11)
Species of the genus Bothrops induce the vast majority of snakebite envenomings in Latin America. A preclinical study was performed in the context of a regional network of public laboratories involved in the production, ...

Neutralization of Bothrops mattogrossensis snake venom from Bolivia: Experimental evaluation of llama and donkey antivenoms produced by caprylic acid precipitation 

Fernández, Gil Patrick; Segura Ruiz, Álvaro; Herrera Vega, María; Velasco, Williams; Solano Trejos, María Gabriela; Gutiérrez, José María; León Montero, Guillermo (2010-02-01)
Polyspecific bothropic/crotalic and bothropic/lachesic antivenoms were produced in Bolivia by immunizing two donkeys with the venoms of Bothrops mattogrossensis and Crotalus durissus terrificus and one llama with the venoms ...
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