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Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom 

Navarro Arias, Diego; Vargas Arroyo, Mariángela; Herrera Vega, María; Segura Ruiz, Álvaro; Gómez Argüello, Aarón; Villalta Arrieta, Mauren; Ramírez Arguedas, Nils Antonio; Williams, David J.; Gutiérrez, José María; León Montero, Guillermo (2016-09-15)
A chicken-derived antivenom (ChDAv) towards taipan snake (Oxyuranus scutellatus) venom was produced by purifying anti-taipan IgY from egg yolks of hens immunized with taipan venom. The productivity, antivenomic profile, ...

A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms 

Otero Patiño, Rafael; Gutiérrez, José María; Rojas Céspedes, Gustavo; Núñez Rangel, Vitelbina; Díaz, Abel; Miranda, E.; Uribe, A. F.; Silva, Juan F.; Ospina, J. G.; Medina, Y.; Toro, María Fabiola; García, Martha E.; León Montero, Guillermo; García, M.; Lizano González, Sergio; De La Torre, J.; Márquez, J.; Mena, Y.; González, N.; Arenas, L. C.; Puzón, A.; Blanco, N.; Sierra, A.; Espinal, M. E.; Arboleda, Margarita; Jiménez, J. C.; Ramírez, P.; Díaz, M.; Guzmán, M. C.; Barros, J.; Henao, S.; Ramírez, A.; Macea, U.; Lozano, R. (1999-06)
A randomized blinded clinical trial was performed in 53 patients bitten by Bothrops sp. and Porthidium sp. in Antioquia and Chocó, Colombia, in order to compare the efficacy and safety of two antivenoms made of whole IgG ...

Comparison between IgG and F(ab′)2 polyvalent antivenoms: neutralization of systemic effects induced by Bothrops asper venom in mice, extravasation to muscle tissue, and potential for induction of adverse reactions 

León Montero, Guillermo; Monge Monge, María; Rojas Umaña, Ermila; Lomonte, Bruno; Gutiérrez, José María (2001-06)
Whole IgG and F(ab′)2 equine-derived polyvalent (Crotalinae) antivenoms, prepared from the same batch of hyperimmune plasma, were compared in terms of neutralization of the lethal and defibrinating activities induced by ...

Pathogenic mechanisms underlying adverse reactions induced by intravenous administration of snake antivenoms 

León Montero, Guillermo; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Gutiérrez, José María (2013-12-15)
Snake antivenoms are formulations of immunoglobulins, or immunoglobulin fragments, purified from the plasma of animals immunized with snake venoms. Their therapeutic success lies in their ability to mitigate the progress ...

Impact of Regional Variation in Bothrops asper Snake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approaches 

Gutiérrez, José María; Sanz, Libia; Flores Díaz, Marietta; Figueroa, Lucía; Madrigal Villalobos, Marvin; Herrera Vega, María; Villalta Arrieta, Mauren; León Montero, Guillermo; Estrada Umaña, Ricardo; Borges, Adolfo; Alape Girón, Alberto; Calvete Chornet, Juan José (2010-10-21)
Intraspecific snake venom variations have implications in the preparation of venom pools for the generation of antivenoms. The impact of such variation in the cross-reactivity of antivenoms against Bothrops asper venom was ...
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Antivenoms for the treatment of snakebite envenomings: The road ahead 

Gutiérrez, José María; León Montero, Guillermo; Burnouf, Thierry (2011-05)
The parenteral administration of antivenoms is the cornerstone of snakebite envenoming therapy. Efforts are made to ensure that antivenoms of adequate efficacy and safety are available world-wide. We address the main issues ...

Anti-human erythrocyte antibodies in horse-derived antivenoms used in the treatment of snakebite envenomations 

León Montero, Guillermo; Rodríguez, Miguel Angel; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María (2007-03)
This work examined the presence of antibodies reacting with human erythrocytes in horse-derived antivenoms used in the treatment of snakebite envenomations, and assessed the efficacy of various fractionation protocols in ...

Comparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venom 

Olmedo, Hidekel; Herrera Vega, María; Rojas Sánchez, Leonardo; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Leiguez, Elbio; Teixeira, Catarina de Fátima; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo; Montero Villalobos, Mavis Lili (2014)
The adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro, most of the venom proteins are similarly ...
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Factors associated with adverse reactions induced by caprylic acid-fractionated whole IgG preparations: comparison between horse, sheep and camel IgGs 

Herrera Vega, María; León Montero, Guillermo; Segura Ruiz, Álvaro; Meneses, Fabricio; Lomonte, Bruno; Chippaux, Jean Philippe; Gutiérrez, José María (2005-12)
Caprylic acid purification of IgG, currently used in the manufacture of horse-derived antivenoms, was successfully adapted for the preparation of sheep and camel IgG. Sheep IgG had a molecular mass of approximately 150 ...
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Antivenomic Characterization of Two Antivenoms Against the Venom of the Taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia 

Herrera Vega, María; Paiva, Owen K.; Pagotto, Ana Helena; Segura Ruiz, Álvaro; Serrano, Solange M. T.; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Jensen, Simon D.; León Montero, Guillermo; Williams, David J.; Gutiérrez, José María (2014-11-05)
Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were ...
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  • Repositorios universitarios

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