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Characterization of events associated with apoptosis/anoikis induced by snake venom metalloproteinase BaP1 on human endothelial cells

Díaz Oreiro, Cecilia; Valverde, Lorena; Brenes García, Oscar Gerardo; Rucavado Romero, Alexandra; Gutiérrez, José María (2005-02)

Human endothelial EA.hy926 cells were incubated with BaP1, a hemorrhagic metalloproteinase purified from Bothrops asper snake venom. Since the first hour of incubation with the proteinase, cells started showing DNA ...

Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom

Bregge Silva, Cristiane; Nonato, Maria Cristina; de Albuquerque, Sérgio; Ho, Paulo Lee; Junqueira de Azevedo, Inácio de Loiola Meirelles; Vasconcelos Diniz, Marcelo Ribeiro; Lomonte, Bruno; Rucavado Romero, Alexandra; Díaz Oreiro, Cecilia; Gutiérrez, José María; Arantes, Eliane Candiani (2012-12-01)

The aim of this study was the isolation of the LAAO from Lachesis muta venom (LmLAAO) and its biochemical, functional and structural characterization. Two different purification protocols were developed and both provided ...

Experimental pathology of local tissue damage induced by Bothrops asper snake venom

Gutiérrez, José María; Rucavado Romero, Alexandra; Chaves Mora, Fernando; Díaz Oreiro, Cecilia; Escalante Muñoz, Teresa (2009-12-01)

Envenomations by Bothrops asper are often associated with complex and severe local pathological manifestations, including edema, blistering, dermonecrosis, myonecrosis and hemorrhage. The pathogenesis of these alterations ...

Modulation of the Susceptibility of Human Erythrocytes to Snake Venom Myotoxic Phospholipases A2: Role of Negatively Charged Phospholipids as Potential Membrane Binding Sites

Díaz Oreiro, Cecilia; León Montero, Guillermo; Rucavado Romero, Alexandra; Rojas Campos, Norman; Schroit, Alan J.; Gutiérrez, José María (2001-07-01)

Cerrophidion (Bothrops) godmani myotoxins I (CGMT-I) and II (CGMT-II), Asp-49 and Lys-49 phospholipases A(2) (PLA2s), which drastically differ in enzymatic activity, were devoid of direct hemolytic effects on erythrocytes ...

Increments in cytokines and matrix metalloproteinases in skeletal muscle after injection of tissue-damaging toxins from the venom of the snake Bothrops asper

Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Teixeira, Catarina de Fátima; Fernandes, Cristina Maria; Díaz Oreiro, Cecilia; Gutiérrez, José María (2002)

Envenomations by the snake Bothrops asper are characterized by prominent local tissue damage (i.e. myonecrosis), blistering, hemorrhage and edema. Various phospholipases A2 and metalloproteinases that induce local pathological ...

Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage

Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Díaz Oreiro, Cecilia (2005-06-15)

Zinc-dependent metalloproteinases are responsible for the hemorrhagic activity characteristic of viperid snake venoms. Snake venom metalloproteinases (SVMPs) are classified in various groups (P-I–IV), according to their ...

Comparison of biochemical and cytotoxic activities of extracts obtained from dorsal spines and caudal fin of adult and juvenile non-native Caribbean lionfish (Pterois volitans/miles)

Ortiz Chaves, Natalia; Lomonte, Bruno; Rucavado Romero, Alexandra; Díaz Oreiro, Cecilia; Sáenz Espinoza, Aránzazu (2017-10)

Pterois volitans/miles lionfish (adult and juvenile) dorsal spines and caudal fin extracts were compared in their general composition, enzymatic activities and hemolytic and cytotoxic effects on bovine aortic endothelial ...