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Comparative study of the efficacy and safety of two polyvalent, caprylic acid fractionated [IgG and F(ab0)2] antivenoms, in Bothrops asper bites in Colombia 

Otero Patiño, Rafael; Segura Ruiz, Álvaro; Pereañez, Jaime Andrés; Herrera Vega, María; Angulo Ugalde, Yamileth; León Montero, Guillermo; Gutiérrez, José María; Barona, Jacqueline; Estrada, Sebastián; Quintana, Juan Carlos; Vargas Muñoz, Leidy Johana; Gómez, Juan Pablo; Díaz, Abel; Suárez, Ana María; Fernández, Jorge; Ramírez, Patricia; Fabra, Patricia E.; Perea, Monica; Fernández, Diego; Arroyo, Yobana; Betancur, Dalila; Pupo, Lady; Córdoba, Elkin; Ramírez, Eugenio; Arrieta, Ana Berta; Rivero, Alcides; Mosquera, Diana Carolina; Conrado, Nectty Lorena; Ortiz, Rosina (2012-02)
The efficacy and safety of two polyvalent horse-derived antivenoms in Bothrops asper envenomings were tested in a randomized, double-blind, clinical trial performed in Colombia. Both antivenoms were manufactured from the ...

Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity 

Herrera Vega, María; Fernández Ulate, Julián; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; León Montero, Guillermo; Angulo Ugalde, Yamileth; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2012-04-03)
The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of ...

Neutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab0)2 and whole IgG antivenoms 

Herrera Vega, María; de Cássia de O. Collaço, Rita; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Wright, Christine E.; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; León Montero, Guillermo; Williams, David J.; Rodrigues Simioni, Lea; Gutiérrez, José María (2016-01-22)
The neuromuscular junction activity of Oxyuranus scutellatus venom and its presynaptic neurotoxin, taipoxin, and their neutralization by two antivenoms were examined in mouse phrenic nerve-diaphragm preparations. The action ...
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Factors associated with adverse reactions induced by caprylic acid-fractionated whole IgG preparations: comparison between horse, sheep and camel IgGs 

Herrera Vega, María; León Montero, Guillermo; Segura Ruiz, Álvaro; Meneses, Fabricio; Lomonte, Bruno; Chippaux, Jean Philippe; Gutiérrez, José María (2005-12)
Caprylic acid purification of IgG, currently used in the manufacture of horse-derived antivenoms, was successfully adapted for the preparation of sheep and camel IgG. Sheep IgG had a molecular mass of approximately 150 ...
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Preclinical evaluation of the efficacy of antivenoms for snakebite envenoming: State-of-the-art and challenges ahead 

Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Sánchez Brenes, Andrés; Sanz, Libia; Lomonte, Bruno; León Montero, Guillermo; Calvete Chornet, Juan José; Gutiérrez, José María (2017-05)
Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. The efficacy of antivenoms to neutralize toxicity of medically-relevant snake venoms has to be demonstrated through meticulous ...

Immune Response Towards Snake Venoms 

León Montero, Guillermo; Sánchez Céspedes, Laura Vanessa; Hernández, Andrés; Villalta Arrieta, Mauren; Herrera Vega, María; Segura Ruiz, Álvaro; Estrada Umaña, Ricardo; Gutiérrez, José María (2011)
The immune response involves a complex repertoire of innate and adaptive responses to foreign agents in the organism. The present review focuses on the immune response to snake venoms, including those occurring in snakebite ...
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Preclinical evaluation of caprylic-acid fractionated IgG antivenom for the treatment of taipan (Oxyuranus scutellatus) envneoming in Papua New Guinea 

Vargas Arroyo, Mariángela; Segura Ruiz, Álvaro; Herrera Vega, María; Villalta Arrieta, Mauren; Estrada Umaña, Ricardo; Cerdas Solís, Maykel; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; León Montero, Guillermo; Gutiérrez, José María; Williams, David J. (PLoS Neglected Tropical Diseases 5 (5) e1144, 2011-05-17)
Background: Snake bite is a common medical emergency in Papua New Guinea (PNG). The taipan, Oxyuranus scutellatus, inflicts a large number of bites that, in the absence of antivenom therapy, result in high mortality. ...
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Intravenous administration of equine-derived whole IgG antivenom does not induce early adverse reactions in non-envenomed horses and cows 

Estrada Umaña, Ricardo; Herrera Vega, María; Segura Ruiz, Álvaro; Araya, Javier; Boschini Figueroa, Carlos; Gutiérrez, José María; León Montero, Guillermo (2010-11)
Administration of antivenoms to treat snakebite envenomings has the potential risk of inducing early adverse reactions. The mechanisms involved in these reactions are unclear. In this study, polyspecific antivenom consisting ...
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Stability of equine IgG antivenoms obtained by caprylic acid precipitation: Towards a liquid formulation stable at tropical room temperature 

Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Herrera Vega, María; González, Esteban; Solano Trejos, María Gabriela; Gutiérrez, José María; León Montero, Guillermo (2009-05)
Liquid formulations of antivenom require a cold chain for their distribution and storage, especially in tropical countries characterized by high temperature and humidity (climatic zone IV). Since cold chain is often deficient ...

Comparison of the effect of Crotalus simus and Crotalus durissus ruruima venoms on the equine antibody response towards Bothrops asper venom: Implications for the production of polyspecific snake antivenoms 

Dos Santos, María Cristina; Arroyo Portilla, Cynthia; Solano Rojas, Sergio; Herrera Vega, María; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo (2011-02)
Antivenoms are preparations of immunoglobulins purified from the plasma of animals immunized with snake venoms. Depending on the number of venoms used during the immunization, antivenoms can be monospecific (if venom from ...
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