Browsing Microbiología by Title
Now showing items 22-41 of 1169
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A Lys49-PLA2 myotoxin of Bothrops asper triggers a rapid death of macrophages that involves autocrine purinergic receptor signaling
(Cell Death and Disease (2012) 3, e343, 2012-07-05)Lys49-PLA2 myotoxins, an important component of various viperid snake venoms, are a class of PLA2-homolog proteins deprived of catalytic activity. Similar to enzymatically active PLA2 (Asp49) and to other classes of ... -
A MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruption
(2016-08)The epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient ... -
A multicomponent strategy to improve the availability of antivenom for treating snakebite envenoming
(2014-07-01)Snakebite envenoming is a common but neglected public health problem, particularly in impoverished rural regions of sub-Saharan Africa, Asia and Latin America. The only validated treatment for this condition is passive ... -
A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
(2018-04-27)Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of ... -
A neutralizing recombinant single chain antibody, scFv, against BaP1, A P-I hemorrhagic metalloproteinase from Bothrops asper snake venom
(2014-09-01)BaP1 is a P-I class snake venom metalloproteinase (SVMP) relevant in the local tissue damage associated with envenomings by Bothrops asper, a medically important snake species in Central America and parts of South and North ... -
A new method for the detection of phospholipase A2 variants: identification of isozymes in the venoms of newborn and adult Bothrops asper (terciopelo) snakes
(1988)A new method for the identification of phospholipase A2 isozymes in snake venoms is described. The technique is based on the separation of the venom components by isoelectric focusing in agarose gels, transfer of the protein ... -
A new muscle damaging toxin, myotoxin II, from the venom of the snake Bothrops asper (terciopelo)
(1989)A new muscle damaging toxin, myotoxin II, was purified from the venom of Bothrops asper by ion-exchange chromatography on CMSephadex C-25. The toxin is a dimeric, basic protein with a monomer mol.wt of 13,341, according ... -
A new Pan African polyspecific antivenom developed in response to the antivenom crisis in Africa
(2003-07)Currently there is a crisis in the supply of antivenom for treatment of snake bite in sub-Saharan Africa. Commercial pressures have resulted in the reduction or even cessation of production of antivenom by European ... -
A novel approach to biological risk surveillance in occupational hygiene: A case study on the use of quantitative microbial risk assessment to determine occupational risks for wastewater treatment plants workers in Costa Rica
(2023)Wastewater treatment is essential for preventing infectious diseases and water pollution. To perform this process, workers operate and maintain wastewater treatment plants (WWTPs); thus, they are exposed to the many pathogens ... -
A novel assay for detecting virus-specific antibodies triggering activation of Fcγ receptors
(2013-01-31)IgG responses are crucial in antiviral defence and instrumental for the serodiagnosis of infections. Fcγ receptors (FcγRs), which recognize the Fc-part of IgG, differ regarding their IgG binding affinity, IgG subclass ... -
A Novel Green Chemistry Method for Nonaqueous Extraction and High-Performance Liquid Chromatography Detection of First-, Second-, and Third-Generation Tetracyclines, 4-Epitetracycline, and Tylosin in Animal Feeds
(2012-06)Although tetracyclines and macrolides are common additives for animal nutrition, methods for their simultaneous determination in animal feeds are nonexistent. By coupling an organic extraction and solid-phase extraction ... -
A novel pentameric phospholipase A2 myotoxin (PophPLA2) from the venom of the pit viper Porthidium ophryomegas
(2018-06-07)The first toxin isolated from the venomous pit viper Porthidiumophryomegas is a basic pentameric phospholipase A2 (PophPLA2). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted ... -
A phospholipase A2 from Bothrops asper snake venom activates neutrophils in culture: Expression of cyclooxygenase-2 and PGE2 biosynthesis
(2011-02)In this study, the production of prostaglandin E2 (PGE2) and up-regulation in cyclooxygenase (COX) pathway induced by a phospholipase A2 (PLA2), myotoxin-III (MT-III), purified from Bothrops asper snake venom, in isolated ... -
A potent antihemorrhagin in the serum of the non-poisonous water snake Natrix tessellata: isolation, characterization and mechanism of neutralization
(1994-12)The main natural antihemorrhagic factor (NtAH), which inhibits the hemorrhagic activity of Bothrops asper snake venom, was isolated from the serum of the non-poisonous water snake Natrix tessellata by ammonium sulfate ... -
A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms
(1999-06)A randomized blinded clinical trial was performed in 53 patients bitten by Bothrops sp. and Porthidium sp. in Antioquia and Chocó, Colombia, in order to compare the efficacy and safety of two antivenoms made of whole IgG ... -
A randomized double-blind clinical trial of two antivenoms in patients bitten by Bothrops atrox in Colombia
(1996-12)A randomized double-blind clinical trial in 39 patients envenomed by Bothrops atrox in Antioquia and Chocó, Colombia, was performed to compare the efficacy and safety of 2 equine-derived antivenoms prepared at Instituto ... -
A review of the genus Rickettsia in Central America
(2018)In this paper, we present a historical review of rickettsiosis in Central America and also the most recent findings of Rickettsia in ectoparasites. All countries of Central America have records of rickettsiosis. Regarding ... -
A secreted phospholipase A2 induces formation of smooth muscle foam cells which trans-differentiate to macrophage-like state
(2019)Vascular smooth muscle cells (VSMCs) loaded with lipid droplets (LDs) are markers of atherosclerosis. In this disease, inflammatory Group IIA-secreted phospholipase A2s (GIIA sPLA2s) are highly expressed in VSMCs, but ... -
A single exposure of sediment sulphate-reducing bacteria to oxytetracycline concentrations relevant to aquaculture enduringly disturbed their activity, abundance and community structure
(2015-05)Aim: although feed medicated with antibiotics is widely used in animal production to prevent and treat bacterial infections, the effect of these drugs on nontarget anaerobic bacteria is unknown. We aimed to clarify whether ... -
A snake venom secreted phospholipase A2 induces foam cell formation depending on the activation of factors involved in lipid homeostasis
(2018-06)MT-III, a snake venom GIIA sPLA2, which shares structural and functional features with mammalian GIIA sPLA2s, activates macrophage defense functions including lipid droplet (LDs) formation, organelle involved in both lipid ...