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Isolation of an acidic phospholipase A2 from the venom of the snake Bothrops asper of Costa Rica: Biochemical and toxicological characterization

Fernández Ulate, Julián; Gutiérrez, José María; Angulo Ugalde, Yamileth; Sanz, Libia; Juárez, Paula; Calvete Chornet, Juan José; Lomonte, Bruno (2010-03)

Phospholipases A2 (PLA2) are major components of snake venoms, exerting a variety of relevant toxic actions such as neurotoxicity and myotoxicity, among others. Since the majority of toxic PLA2s are basic proteins, acidic ...

Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae)

Fernández Ulate, Julián; Alape Girón, Alberto; Angulo Ugalde, Yamileth; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José; Lomonte, Bruno (2011-01-31)

The proteome of the venom of Micrurus nigrocinctus (Central American coral snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein composition. Comparative ...

Venomous snakes of Costa Rica: biological and medical implications of their venom proteomic profiles analyzed through the strategy of snake venomics

Lomonte, Bruno; Fernández Ulate, Julián; Sanz, Libia; Angulo Ugalde, Yamileth; Sasa Marín, Mahmood; Gutiérrez, José María; Calvete Chornet, Juan José (Journal of Proteomics XX (2014) XXX – XXX, Available online 24 February 2014, 2014-02-24)

In spite of its small territory of ~50,000 km2, Costa Rica harbors a remarkably rich biodiversity. Its herpetofauna includes 138 species of snakes, of which sixteen pit vipers (family Viperidae, subfamily Crotalinae), five ...

Isolation and characterization of four medium-size disintegrins from the venoms of Central American viperid snakes of the genera Atropoides, Bothrops, Cerrophidion and Crotalus

Angulo Ugalde, Yamileth; Castro Godínez, Adriana; Lomonte, Bruno; Rucavado Romero, Alexandra; Fernández Ulate, Julián; Calvete Chornet, Juan José; Gutiérrez, José María (2014-12)

Four disintegrins were isolated from the venoms of the Central American viperid snakes Atropoides mexicanus (atropoimin), Bothrops asper (bothrasperin), Cerrophidion sasai (sasaimin), and Crotalus simus (simusmin). ...

Snake Venomics and Antivenomics of the Arboreal Neotropical Pitvipers Bothriechis lateralis and Bothriechis schlegelii

Lomonte, Bruno; Escolano, José; Fernández Ulate, Julián; Sanz, Libia; Angulo Ugalde, Yamileth; Gutiérrez, José María; Calvete Chornet, Juan José (2008-04-30)

We report the comparative proteomic characterization of the venoms of two related neotropical arboreal pitvipers from Costa Rica of the genus Bothriechis, B. lateralis (side-striped palm pit viper) and B. schlegelii (eyelash ...

Snake Venomics of the Lesser Antillean Pit Vipers Bothrops caribbaeus and Bothrops lanceolatus: Correlation with Toxicological Activities and Immunoreactivity of a Heterologous Antivenom

Gutiérrez, José María; Sanz, Libia; Escolano, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Rucavado Romero, Alexandra; Warrell, David A.; Calvete Chornet, Juan José (2008-10)

The venom proteomes of the snakes Bothrops caribbaeus and Bothrops lanceolatus, endemic to the Lesser Antillean islands of Saint Lucia and Martinique, respectively, were characterized by reversephase HPLC fractionation, ...

Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest

Gonçalves Machado, Larissa; Pla Ferrer, Davinia; Sanz, Libia; Jorge, Roberta Jeane B.; Leitão De Araújo, Moema; Alves, Maria Lúcia M.; Alvares, Diego Janisch; De Miranda, Joari; Nowatzki, Jenifer; de Morais Zani, Karen; Fernandes, Wilson; Tanaka Azevedo, Anita Mitico; Fernández Ulate, Julián; Zingali, Russolina B.; Gutiérrez, José María; Corrêa Netto, Carlos; Calvete Chornet, Juan José (2016-03-01)

Bothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern Brazil, Paraguay, and northern Argentina (Misiones). Within its geographic range, ...

Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities

Antúnez Oliva, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Sanz, Libia; Pérez, Alicia; Calvete Chornet, Juan José; Gutiérrez, José María (2010-09-30)

Viperid snakes of the genus Atropoides are distributed in Mexico and Central America and, owing to their size and venom yield, are capable of provoking severe envenomings in humans. This study evaluated, using an ‘antivenomics’ ...

Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids

Fernández Ulate, Julián; Vargas Vargas, Nancy; Pla Ferrer, Davinia; Sasa Marín, Mahmood; Rey Suárez, Paola; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José; Lomonte, Bruno (2015-12-01)

Protein composition, toxicity, and neutralization of the venoms of Micrurus alleni and Micrurus mosquitensis, two sympatric monadal coral snakes found in humid environments of the Caribbean region of Costa Rica, were ...

First crotoxin-like phospholipase A2 complex from a New World non-rattlesnake species: Nigroviriditoxin, from the arboreal Neotropical snake Bothriechis nigroviridis

Lomonte, Bruno; Mora Obando, Diana; Fernández Ulate, Julián; Sanz, Libia; Pla Ferrer, Davinia; Gutiérrez, José María; Calvete Chornet, Juan José (Toxicon vol 93:144–154, 2015-01)

Bothriechis nigroviridis is an arboreal Neotropical pitviper found in Costa Rica and Panam a. A previous proteomic profiling of its venom revealed the presence of proteins with homology to the A and B subunits of ...