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Preclinical assessment of the neutralizing capacity of antivenoms produced in six Latin American countries against medically-relevant Bothrops snake venoms 

Segura Ruiz, Álvaro; Castillo, M. C.; Núñez Rangel, Vitelbina; Yarlequé, A.; Gonçalves, Luís Roberto de Camargo; Villalta Arrieta, Mauren; Bonilla, César; Herrera Vega, María; Fernández, M.; Yano, M. Y.; Araújo, H. P.; Boller, M. A. A.; León, P.; Sano Martins, I. S.; Gómez, A.; Fernández, Gil Patrick; Geoghegan, Patricia; Higashi, Hisako Gondo; León Montero, Guillermo; Gutiérrez, José María; Tintaya, Benigno (2010-11)
Species of the genus Bothrops induce the vast majority of snakebite envenomings in Latin America. A preclinical study was performed in the context of a regional network of public laboratories involved in the production, ...

Inflammatory events induced by Lys-49 and Asp-49 phospholipases A2 isolated from Bothrops asper snake venom: role of catalytic activity 

Zuliani, Juliana Pavan; Fernandes, Cristina Maria; Zamunér, Stella Regina; Gutiérrez, José María; Teixeira, Catarina de Fátima (2005-03)
The inflammatory events induced in the peritoneal cavity of mice by two PLA2s isolated from Bothrops asper snake venom were investigated. MT-III, an Asp-49 catalytically active enzyme and MT-II, a catalytically inactive ...

A new method for the detection of phospholipase A2 variants: identification of isozymes in the venoms of newborn and adult Bothrops asper (terciopelo) snakes 

Moreno Robles, Edgardo; Alape Girón, Alberto; Sánchez, Manuel; Gutiérrez, José María (1988)
A new method for the identification of phospholipase A2 isozymes in snake venoms is described. The technique is based on the separation of the venom components by isoelectric focusing in agarose gels, transfer of the protein ...

The Global Snake Bite Initiative: an antidote for snake bite 

Williams, David J.; Gutiérrez, José María; Harrison, Robert A.; Warrell, David A.; White, Julian; Winkel, Kenneth D.; Gopalakrishnakone, Ponnampalam (2010-01-02)
Clinicians have for a long time witnessed the tragedy of injury, disability, and death from snake bite that is a daily occurrence in many parts of Africa, Asia, and Latin America. To many people living in these ...

Inhibition of local hemorrhage and dermonecrosis induced by Bothrops asper snake venom: effectiveness of early in situ administration of the peptidomimetic metalloproteinase inhibitor batimastat and the chelating agent CaNa2EDTA 

Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Franceschi, Aida; Chaves Mora, Fernando; León Montero, Guillermo; Cury, Yara; Ovadia, Michael; Gutiérrez, José María (2000-11)
The effectiveness of the chelating agent CaNa2EDTA and the peptidomimetic matrix metalloproteinase inhibitor batimastat (BB-94) to inhibit local tissue damage induced by Bothrops asper snake venom was studied in mice. Both ...

Pharmacokinetic-pharmacodynamic relationships of immunoglobulin therapy for envenomation. 

Gutiérrez, José María; León Montero, Guillermo; Lomonte, Bruno (2003-07)
Parenteral administration of horse- and sheep-derived antivenoms constitutes the cornerstone in the therapy of envenomations induced by animal bites and stings. Depending on the type of neutralising molecule, antivenoms ...
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Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: Comparison with two commercial antivenoms 

Otero Patiño, Rafael; Núñez Rangel, Vitelbina; Gutiérrez, José María; Robles, Abel; Estrada Umaña, Ricardo; Osorio, Raúl Guillermo; Del Valle, G.; Valderrama, R.; Giraldo, Cesar Augusto (1997-04)
Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atr-ox from Antioquia and Choco (Colombia). ...

Degenerative and regenerative changes in murine skeletal muscle after injection of venom from the snake Bothrops asper: a histochemical and immunocytochemical study. 

Arce, Vilma; Brenes Pino, Fernando; Gutiérrez, José María (1991)
The degenerative and regenerative changes in murine skeletal muscle after injection of Bothrops asper venom were studied by histological, lectin histochemical and immunocytochemical techniques. According to our observations, ...
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Venom Composition and Diet of the Cantil Agkistrodon bilineatus howardgloydi (Serpentes: Viperidae) 

Solórzano López, Alejandro; Romero, Marjorie; Gutiérrez, José María; Sasa Marín, Mahmood (1999-12)
The southernmost subspecies of the cantil, Agkistrodon bilineatus howardgloydi, occurs in almost all terrestrial habitats of the sector Santa Rosa National Park, in Guanacaste Conservation Area, Costa Rica. We obtained 21 ...

A Lys49 phospholipase A2 homologue from Bothrops asper snake venom induces proliferation, apoptosis and necrosis in a lymphoblastoid cell line 

Mora Rodríguez, Rodrigo Antonio; Valverde Rojas, Berta E.; Díaz Oreiro, Cecilia; Lomonte, Bruno; Gutiérrez, José María (2005-04)
Lys49 phospholipase A2 homologues are abundant in viperid snake venoms. These proteins have substitutions at the calcium-binding loop and catalytic center which render them enzymatically inactive; however, they display a ...
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