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dc.creatorBolaños Villegas, Pablo Alberto
dc.creatorYang, Xiaohui
dc.creatorWang, Huei-Jing
dc.creatorJuan, Chien-Ta
dc.creatorChuang, Min-Hsiang
dc.creatorMakaroff, Christopher A.
dc.creatorJauh, Guang-Yuh
dc.descriptionartículo -- Universidad de Costa Rica. Escuela de Agronomía, 2013.es_ES
dc.description.abstractThe proper transmission of DNA in dividing cells is crucial for the survival of eukaryotic organisms. During cell division, faithful segregation of replicated chromosomes requires their tight attachment, known as sister chromatid cohesion, until anaphase. Sister chromatid cohesion is established during S-phase in a process requiring an acetyltransferase that in yeast is known as Establishment of cohesion 1 (Eco1). Inactivation of Eco1 typically disrupts chromosome segregation and homologous recombination-dependent DNA repair in dividing cells, ultimately resulting in lethality. We report here the isolation and detailed characterization of two homozygous T-DNA insertion mutants for the Arabidopsis thaliana Eco1 homolog, CHROMOSOME TRANSMISSION FIDELITY 7/ESTABLISHMENT OF COHESION 1 (CTF7/ECO1), called ctf7-1 and ctf7-2. Mutants exhibited dwarfism, poor anther development and sterility. Analysis of somatic tissues by flow cytometry, scanning electron microscopy and quantitative real-time PCR identified defects in DNA repair and cell division, including an increase in the area of leaf epidermal cells, an increase in DNA content and the upregulation of genes involved in DNA repair including BRCA1 and PARP2. No significant change was observed in the expression of genes that influence entry into the endocycle. Analysis of meiocytes identified changes in chromosome morphology and defective segregation; the abundance of chromosomal-bound cohesion subunits was also reduced. Transcript levels for several meiotic genes, including the recombinase genes DMC1 and RAD51C and the S-phase licensing factor CDC45 were elevated in mutant anthers. Taken together our results demonstrate that Arabidopsis CTF7/ECO1 plays important roles in the preservation of genome integrity and meiosis.es_ES
dc.description.sponsorshipThis work was supported by research grants from Academia Sinica (Taiwan), the National Science and Technology Program for Agricultural Biotechnology (NSTP/AB, 098S0030055-AA, Taiwan) and the National Science Council (99-2321-B-001-036-MY3) to G-YJ by a grant (MCB0718191) to CAM from the National Science Foundation (NSF) and by a study abroad contract to PBV from the University of Costa Rica.es_ES
dc.publisherThe Plant Journal 75: 927–940es_ES
dc.rightsAtribución-NoComercial 3.0 Internacionales_ES
dc.subjectArabidopsis thalianaes_ES
dc.subjectGenética vegetales_ES
dc.subjectBiología celulares_ES
dc.titleArabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosises_ES
dc.typeartículo científicoes_ES
dc.identifier.doidoi: 10.1111/tpj.12261es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Agronomíaes_ES

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Atribución-NoComercial 3.0 Internacional
Except where otherwise noted, this item's license is described as Atribución-NoComercial 3.0 Internacional