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Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
| dc.creator | Bolaños Villegas, Pablo Alberto | |
| dc.creator | Yang, Xiaohui | |
| dc.creator | Makaroff, Christopher A. | |
| dc.creator | Jauh, Guang-Yuh | |
| dc.date.accessioned | 2015-02-24T21:43:55Z | |
| dc.date.available | 2015-02-24T21:43:55Z | |
| dc.date.issued | 2014-04-05 | |
| dc.identifier.citation | http://www.bio-protocol.org/e1102 | |
| dc.identifier.uri | https://hdl.handle.net/10669/11313 | |
| dc.description | artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014 | es_ES |
| dc.description.abstract | This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes. | es_ES |
| dc.description.sponsorship | This work was supported by research grants from Academia Sinica (Taiwan), the National Science and Technology Program for Agricultural Biotechnology (NSTP/AB, 098S0030055-AA, Taiwan) and the National Science Council (99-2321-B-001-036-MY3) to G-YJ, by a grant (MCB0718191) to CAM from the National Science Foundation (NSF) and by a study abroad contract to PBV from the University of Costa Rica. | es_ES |
| dc.language.iso | en_US | es_ES |
| dc.publisher | Bio-protocol 4(7), e1102, 2014 | es_ES |
| dc.subject | Meiosis | es_ES |
| dc.subject | Arabidopsis thaliana | es_ES |
| dc.subject | Cromosomas | es_ES |
| dc.subject | Hibridización in situ fluorescente | es_ES |
| dc.title | Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization | es_ES |
| dc.type | artículo original | |
| dc.description.procedence | UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Agronomía | es_ES |
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Biología [1635]