Isolation and partial characterization of Lachesis muta melanocephala coagulant proteinase: biochemical parameters of the venom

Aragón Ortiz, Federico

dc.creator	Aragón Ortiz, Federico
dc.date	2016-04-18
dc.date.accessioned	2016-05-03T15:32:46Z
dc.date.available	2016-05-03T15:32:46Z
dc.identifier	http://revistas.ucr.ac.cr/index.php/rbt/article/view/23819
dc.identifier	10.15517/rbt.v36i2B.23819
dc.identifier.uri	https://hdl.handle.net/10669/27324
dc.description	The coagulant proteinase of L. m. melanocephala was purified by DEAE Sephadex A-50 followed by agmatine CH- Sepharose and gel filtration on Sephadex G-100. The enzyme exhibited many of the properties adscribed to "mudasa", the coagulant proteinase from L. m. stenophirs venom. Its molecular weight by gel filtration was 35 kDa and its specific clotting activity was 702 NIH/mg of protein. A 32 fold increase in the clotting activity was obtained by purification. The coagulant proteinase exhibited esterolytic activities toward lysine and arginine esters as well as amidolytic activity. Significant differences are observed when compared with the activities of "mudasa", the former is less esterolytic and amidolytic, although its activity toward TLEME is higher. Significant differences in the activities are also observed when the venom from the Pacific and Atlantic L. muta populations (corresponding to the subspecies L. m. melanocefala and L. m. stenophyrs) are compared toward the same substrates. The Pacific type is less amidolytic and more esterolytic toward BAME and BAEE, although toward the lysine and tyrosine esters no significant differences are observed. The vemon from the Pacific population is more coagulant and less proteolytic than the venom from the Atlantic population. Analytical isoelectric focusing of both populations of venom revealed important differences in the number and intensity of the protein bands. The results here given further substantiate the taxonomical differentiation already given to the Pacific and Atlantic Costa Rican population of L. muta.	en-US
dc.format	application/pdf
dc.language	eng
dc.publisher	Universidad de Costa Rica	en-US
dc.rights	Copyright (c) 2016 Revista de Biología Tropical/International Journal of Tropical Biology and Conservation	en-US
dc.source	Revista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol 36, No 2B (1988); 387-392	en-US
dc.source	Revista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol 36, No 2B (1988); 387-392	es-ES
dc.source	Revista Biología Tropical; Vol 36, No 2B (1988); 387-392	pt-PT
dc.source	2215-2075
dc.source	0034-7744
dc.source	10.15517/rbt.v36i2B
dc.title	Isolation and partial characterization of Lachesis muta melanocephala coagulant proteinase: biochemical parameters of the venom	en-US
dc.type	artículo original

Ficheros en el ítem

Ficheros	Tamaño	Formato	Ver
No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Revista de Biología Tropical [2107]

Mostrar el registro sencillo del ítem