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dc.creatorFernández Culma, Maritza
dc.creatorPereañez, Jaime Andrés
dc.creatorNúñez Rangel, Vitelbina
dc.creatorLomonte, Bruno
dc.date.accessioned2014-02-10T20:07:04Z
dc.date.available2014-02-10T20:07:04Z
dc.date.issued2014-01-22
dc.identifier.citationhttps://peerj.com/articles/246/es
dc.identifier.issn2167-8359
dc.identifier.urihttp://hdl.handle.net/10669/9001
dc.descriptionartículo (arbitrado)--Universidad de Costa Rica, Instituto Clodomiro Picado. 2014es
dc.description.abstractBothrops punctatus is an endangered, semi-arboreal pitviper species distributed in Panam´a, Colombia, and Ecuador, whose venom is poorly characterized. In the present work, the protein composition of this venom was profiled using the ‘snake venomics’ analytical strategy. Decomplexation of the crude venomby RP-HPLC and SDS-PAGE, followed by tandemmass spectrometry of tryptic digests, showed that it consists of proteins assigned to at least nine snake toxin families.Metalloproteinases are predominant in this secretion (41.5% of the total proteins), followed by C-type lectin/lectin-like proteins (16.7%), bradykinin-potentiating peptides (10.7%), phospholipases A2 (9.3%), serine proteinases (5.4%), disintegrins (3.8%), L-amino acid oxidases (3.1%), vascular endothelial growth factors (1.7%), and cysteinerich secretory proteins (1.2%). Altogether, 6.6% of the proteins were not identified. In vitro, the venom exhibited proteolytic, phospholipase A2, and L-amino acid oxidase activities, as well as angiotensin-converting enzyme (ACE)-inhibitory activity, in agreement with the obtained proteomic profile. Cytotoxic activity onmurine C2C12 myoblasts was negative, suggesting that the majority of venom phospholipases A2 likely belong to the acidic type, which often lack major toxic effects. The protein composition of B. punctatus venom shows a good correlation with toxic activities here and previously reported, and adds further data in support of the wide diversity of strategies that have evolved in snake venoms to subdue prey, as increasingly being revealed by proteomic analyses.es
dc.description.sponsorshipUniversidad de Antioquia Sostenibilidad (2013–2014). Vicerrector´ıa de Investigación, Universidad de Costa Rica.es
dc.language.isoen_USes
dc.publisherPeerJ 2:e246es
dc.subjectSnake venomes
dc.subjectViperidaees
dc.subjectproteomicses
dc.subjectBothrops punctatuses
dc.titleSnake venomics of Bothrops punctatus, a semi-arboreal pitviper species from Antioquia, Colombiaes
dc.typeinfo:eu-repo/semantics/articlees
dc.typeArtículo científicoes
dc.identifier.doiDOI 10.7717/peerj.246
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es


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