Snake venomics of Bothrops punctatus, a semi-arboreal pitviper species from Antioquia, Colombia
Fernández Culma, Maritza
Pereañez, Jaime Andrés
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Bothrops punctatus is an endangered, semi-arboreal pitviper species distributed in Panam´a, Colombia, and Ecuador, whose venom is poorly characterized. In the present work, the protein composition of this venom was profiled using the ‘snake venomics’ analytical strategy. Decomplexation of the crude venomby RP-HPLC and SDS-PAGE, followed by tandemmass spectrometry of tryptic digests, showed that it consists of proteins assigned to at least nine snake toxin families.Metalloproteinases are predominant in this secretion (41.5% of the total proteins), followed by C-type lectin/lectin-like proteins (16.7%), bradykinin-potentiating peptides (10.7%), phospholipases A2 (9.3%), serine proteinases (5.4%), disintegrins (3.8%), L-amino acid oxidases (3.1%), vascular endothelial growth factors (1.7%), and cysteinerich secretory proteins (1.2%). Altogether, 6.6% of the proteins were not identified. In vitro, the venom exhibited proteolytic, phospholipase A2, and L-amino acid oxidase activities, as well as angiotensin-converting enzyme (ACE)-inhibitory activity, in agreement with the obtained proteomic profile. Cytotoxic activity onmurine C2C12 myoblasts was negative, suggesting that the majority of venom phospholipases A2 likely belong to the acidic type, which often lack major toxic effects. The protein composition of B. punctatus venom shows a good correlation with toxic activities here and previously reported, and adds further data in support of the wide diversity of strategies that have evolved in snake venoms to subdue prey, as increasingly being revealed by proteomic analyses.
Enlace externo al ítemDOI 10.7717/peerj.246
artículo (arbitrado)--Universidad de Costa Rica, Instituto Clodomiro Picado. 2014