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dc.creatorBarrantes Jiménez, Kenia
dc.creatorAchí Araya, María Rosario
dc.creatorMcCoy, Clyde B.
dc.date.accessioned2014-01-10T21:49:43Z
dc.date.available2014-01-10T21:49:43Z
dc.date.issued2010
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/10669/8958
dc.descriptionartículo (arbitrado)--Universidad de Costa Rica.Instituto de Investigaciones en Salud, 2010es
dc.description.abstractA Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flexneri concentrations (from 10 CFU/ml to 107 CFU/ml). DNA was extracted directly from lettuce after inoculation (direct-PCR) and after an enrichment step (enrichment PCR). Multiplex PCR detection limit was 104 CFU/ml, diagnostic sensitivity and specificity were 100% accurate. An internal amplification control (IAC) of 100 bp was used in order to avoid false negative results. This method produced results in 1 to 2 days while the conventional culture method required 5 to 6 days. Also, the culture method detection limit was 106 CFU/ml, diagnostic sensitivity was 53% and diagnostic specificity was 100%. In this study a Multiplex PCR method for detection of virulence genes in Shigella and EIEC was shown to be effective in terms of diagnostic sensitivity, detection limit and amount of time as compared to Shigella conventional culture.es
dc.description.sponsorshipUniversidad de Costa Ricaes
dc.language.isoen_USes
dc.publisherBrazilian Journal of Microbiology (2010) 41: 993-1000es
dc.subjectShigellaes
dc.subjectBacteriaes
dc.subjectMétodos de Alimentaciónes
dc.subjectSativaes
dc.subjectContaminantees
dc.titleDetection of shigella in lettuce by the use of a rapid molecular assay with increased sensitivityes
dc.typeArtículo científicoes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1590/S1517-838220100004000018
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA)es


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