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dc.creatorMesén Porras, Esteve Alonso
dc.creatorDahdouh Cabia, Sergio
dc.creatorJiménez Quirós, Catherine
dc.creatorMora Castro, Rebeca
dc.creatorRodríguez Sánchez, César
dc.creatorPinto Tomás, Adrián A.
dc.date.accessioned2020-05-19T15:58:49Z
dc.date.available2020-05-19T15:58:49Z
dc.date.issued2020
dc.identifier.citationhttps://revistas.ucr.ac.cr/index.php/agromeso/article/view/36573es_ES
dc.identifier.urihttp://hdl.handle.net/10669/81052
dc.description.abstractThe coffee berry borer (Hypothenemus hampei Ferrari, CBB) is one of the most devastating pests on coffee plantations around the world. Although CBB is susceptible to the effect of δ–endotoxins of Bacillus thuringiensis subs. israelensis (Bti) at laboratory level, the efficacy of this control method is poor in the field, presumably due to the inactivation by digestive proteases different to those required for protoxin activation. Objective. To study whether the addition of a soybean flour extract enriched with protease inhibitors (PI), mixed with Bti crystals and spores (Bti-sc) in an artificial diet, could improve the toxicity of Bti against CBB. Materials and methods. This study was performed in San José, Costa Rica, between 2012 and 2013. A set of adult female CBB insects was exposed to a mixture containing different concentrations of a partially purified soybean meal extract with active PI and lyophilized Bti-sc, and were tested through a bioassay in artificial diet to estimate the sub-lethal concentration (LC50). The mortality results were validated by observing the dissected midgut, whose ultrastructure was analyzed by transmission electron microscopy. Results. The soybean extracts partially degraded the Bti-sc complex, it reduced its LC50 by almost four times (from 1.135 to 0.315 μg μl-1) and enhanced CBB mortality in a concentration-dependent manner. Histological analyses of the midgut confirmed this synergistic effect, since severe epithelial damage to the intestinal epithelium of CBB exposed to Bti-sc + PI was visualized compared to Bti-sc alone. Conclusions. The combination of a soybean extract enriched in PI and Bti-sc enhanced the mortality effect over CBB, which was confirmed by the midgut collapse. Soybean flour is a cost-effective supplement that could increase Bti effectiveness against CBB and delay the appearance of biological resistance.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[801-A6-607]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[801-A2-521]/UCR/Costa Ricaes_ES
dc.description.sponsorshipAgroquímica Industrial RIMAC S.A./[]//Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceAgronomía Mesoamericana, vol.31(2), pp.461-478es_ES
dc.subjectBiological controles_ES
dc.subjectBacterial toxinses_ES
dc.subjectSoybean meales_ES
dc.subjectProtease inhibitorses_ES
dc.subjectSynergismes_ES
dc.titleSoybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampeies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.15517/am.v31i2.36573
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Estructuras Microscópicas (CIEMIC)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Ciencia e Ingeniería de Materiales (CICIMA)es_ES
dc.identifier.codproyecto801-A6-607
dc.identifier.codproyecto801-A2-521


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