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dc.creatorAlvarado Gámez, Ana Lorena
dc.creatorArcos Martínez, María Julia
dc.date.accessioned2020-02-28T15:38:09Z
dc.date.available2020-02-28T15:38:09Z
dc.date.issued2017
dc.identifier.citationhttps://www.sensorsportal.com/HTML/DIGEST/P_2915.htmes_ES
dc.identifier.issn2306-8515
dc.identifier.issn1726-5479
dc.identifier.urihttp://hdl.handle.net/10669/80661
dc.description.abstractIt was developed an amperometric biosensor to determine tungsten in water, based on the inhibition of laccase enzyme, by tungsten ions using pyrocathecol as a substrate. The enzyme was immobilized with a proper mixture containing, bovine serum albumin, and glutaraldehyde, for a cross-linking process over screen-printed carbon electrodes, previously modified with tetrathiafulvalen and gold nanoparticles. Optimized experimental conditions are: pyrocatechol in cell 0.040 mM in a phosphate buffer pH 6.5 and applied potential +350 mV. The repeatability and reproducibility, in terms of relative standard deviation values, of de developed biosensor were 3.3 % (n=3), and 2.2 % (n = 5) respectively, and detection limit was 1.8 × 10-7 mol L-1. Additionally it was determined the kinetics of the systems by means of Michaelis-Menten Km apparent constants, calculated using Lineweaver-Burk plots, with and without tungsten. Kinetic study resembles to be competitive inhibition. A recovery study was performed with spiked blanks with a tungsten certified reference standard, traceable to NIST, giving as a result 102.3 ± 6.7 %; tap water samples analyzed presented a mean concentration of 1.75 µM, and recovery of the tungsten certified reference standard on the tap water samples gave 98.8 ± 3.1 %.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[804-B5-164]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceSensors & Transducers, vol. 211(4), pp.1-7es_ES
dc.subjectBiosensores_ES
dc.subjectCathecoles_ES
dc.subjectGold nanoparticleses_ES
dc.subjectLaccasees_ES
dc.subjectScreen printed carbon electrodeses_ES
dc.subjectTetrathiafulvalenes_ES
dc.subjectTungstenes_ES
dc.subjectWateres_ES
dc.titleModified Laccase-Gold Nanoparticles-Tetrathiafulvalene-SPCEs Based Biosensor to Determine W(VI) in Wateres_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)es_ES
dc.identifier.codproyecto804-B5-164


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