dc.creator | Díaz Oreiro, Cecilia | |
dc.creator | Rivera Hidalgo, Jennifer | |
dc.creator | Lomonte, Bruno | |
dc.creator | Bonilla Murillo, Fabián | |
dc.creator | Diego García, Elia | |
dc.creator | Camacho Umaña, Erika | |
dc.creator | Tytgat, Jan | |
dc.creator | Sasa Marín, Mahmood | |
dc.date.accessioned | 2020-01-30T13:34:18Z | |
dc.date.available | 2020-01-30T13:34:18Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | https://www.sciencedirect.com/science/article/pii/S0041010119304714?via%3Dihub | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/80393 | |
dc.description.abstract | In this study, we characterize the venom of Centruroides edwardsii, one of the most abundant scorpions in urban
and rural areas of Costa Rica, in terms of its biochemical constituents and their biological activities. C. edwardsii
venom is rich in peptides but also contains some higher molecular weight protein components. No phospholipase
A2, hemolytic or fibrinogenolytic activities were found, but the presence of proteolytic and hyaluronidase enzymes
was evidenced by zymography.
Venom proteomic analysis indicates the presence of a hyaluronidase, several cysteine-rich secretory proteins,
metalloproteinases and a peptidylglycine α-hydroxylating monooxygenase like-enzyme. It also includes peptides
similar to the Kþ-channel blocker margatoxin, a dominant toxin in the venom of the related scorpion
C. margaritatus. MS and N-terminal sequencing analysis also reveals the presence of Naþ-channel-modulating
peptides with sequence similarity to orthologs present in other scorpion species of the genera Centruroides and
Tityus. We purified the hyaluronidase (which co-eluted with an allergen 5-like CRiSP) and sequenced ~60% of
this enzyme. We also sequenced some venom gland transcripts that include other cysteine-containing peptides
and a Non-Disulfide Bridged Peptide (NDBP).
Our in vivo experiments characterizing the effects on potential predators and prey show that C. edwardsii
venom induces paralysis in several species of arthropods and geckos; crickets being the most sensitive and
cockroaches and scorpions the most resistant organisms tested. Envenomation signs were also observed in mice,
but no lethality was reached by intraperitoneal administration of this venom up to 120 μg/g body weight. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[741-A5-048]/UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon, vol.171, pp.7-19 | es_ES |
dc.subject | Scorpion venom | es_ES |
dc.subject | Peptides | es_ES |
dc.subject | Hyaluronidase | es_ES |
dc.subject | Paralysis | es_ES |
dc.subject | Toxins | es_ES |
dc.title | Venom characterization of the bark scorpion Centruroides edwardsii (Gervais 1843): composition, biochemical activities and in vivo toxicity for potential prey | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.toxicon.2019.09.021 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicina | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biología | es_ES |
dc.identifier.codproyecto | 741-A5-048 | |