Universidad de Costa Rica
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo Depositar
  • Políticas
  • Contacto
    • español
    • English
  • English 
    • español
    • English
  • Login
View Item 
  •   Kérwá Home
  • Investigación
  • Ciencias básicas
  • Oceanografía y áreas costeras
  • View Item
  •   Kérwá Home
  • Investigación
  • Ciencias básicas
  • Oceanografía y áreas costeras
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Using eDNA to determine the source of organic carbon in seagrass meadows

Artículo científico
View/Open
Artículo principal (883.9Kb)
Date
2017
Author
Reef, Ruth
Atwood, Trisha B.
Samper Villarreal, Jimena
Adame, Maria Fernanda
Sampayo, Eugenia M.
Lovelock, Catherine E.
Metadata
Show full item record
Abstract
Seagrasses sequester globally significant amounts of carbon (C), which is stored mainly in the sediment. BothC fixation by the seagrass (autochthonous) and the trapping of organic matter that is derived from outside theecosystem (allochthonous)contribute to seagrass sediment organic C (OC). However, due to limitations incurrent methods we do not yet fully understand which sources of C are most important to blue C ecosystems.We used environmental-DNA (eDNA) to identify and estimate the contribution of floral sources to seagrass sedi-ment OC sampled from three sites in Moreton Bay, Australia that differ in potential levels of autochthonous andallochthonous OC inputs. Using the plant barcode generbcLand a next-generation sequencing platform we iden-tified 150 plant operational taxonomic units in the sediment samples. We found that seagrass DNA composedon average 88% of the eDNA pool in the sediment samples. Allochtonous sources were primarily mangrove, salt-marsh, and freshwater marsh species, but the proportional contribution of other sources varied among meadows.Carbon and nitrogen (N) stable isotope mixing models suggested a lower autochthonous contribution than theeDNA data, but had high ambiguity due to indistinguishable isotopic values among some of the sources. Ourstudy shows that eDNA can be an effective tool for identifying sources to sediment OC in blue C ecosystems andcan be used in conjunction with stable isotope analysis to reduce ambiguity. Identifying the sources of OC to thesediments of blue C ecosystems is crucial for building robust and accurate C budgets for these systems.
URI
http://hdl.handle.net/10669/78900
External link to the item
10.1002/lno.10499
https://aslopubs.onlinelibrary.wiley.com/doi/full/10.1002/lno.10499
Collections
  • Oceanografía y áreas costeras [100]



  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
  • Repositorio de Fotografías
  • Colección de videos de UPA-VAS
  • Sitios recomendados

  • Buscador regional de LA Referencia
  • Buscador del Open ROAR
  • Scientific Electronic Library Online (SciELO)
  • Directory of Open Access Journals (DOAJ)
  • Redalyc
  • Redes sociales

  • facebook.com/repositoriokerwa
  • @Ciencia_UCR
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo depositar
  • Políticas
Contact Us | Send Feedback
Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
Universidad de Costa Rica
 

 

Browse

All of KérwáCommunities & CollectionsTitlesAuthorsSubjectsProcedenceTypeThis CollectionTitlesAuthorsSubjectsProcedenceType

My Account

LoginRegister

  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
  • Repositorio de Fotografías
  • Colección de videos de UPA-VAS
  • Sitios recomendados

  • Buscador regional de LA Referencia
  • Buscador del Open ROAR
  • Scientific Electronic Library Online (SciELO)
  • Directory of Open Access Journals (DOAJ)
  • Redalyc
  • Redes sociales

  • facebook.com/repositoriokerwa
  • @Ciencia_UCR
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo depositar
  • Políticas
Contact Us | Send Feedback
Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
Universidad de Costa Rica