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dc.creatorJiménez García, Víctor
dc.creatorBangerth, Fritz K.
dc.date.accessioned2019-06-13T15:58:52Z
dc.date.available2019-06-13T15:58:52Z
dc.date.issued2001
dc.identifier.citationhttps://onlinelibrary.wiley.com/doi/full/10.1034/j.1399-3054.2001.1110317.xes_ES
dc.identifier.issn1399-3054
dc.identifier.urihttp://hdl.handle.net/10669/77440
dc.description.abstractCarrot (Daucus carota L. F1 hybrid Starca) excised hypocotyls were cultured on Murashige and Skoog medium with and without 2,4‐dichlorophenoxy acetic acid (2,4‐D) to determine the effect of this plant growth regulator on their further development and their endogenous hormone levels. Culture in the absence of 2,4‐D stimulated root development at one end of the hypocotyl segments and increased the endogenous levels of free indole‐3‐acetic acid (IAA), zeatin/zeatin riboside and N6(Δ2‐isopentenyl) adenine/N6(Δ2‐isopentenyl) adenosine, as determined by radio‐immunoassay. On the other hand, the presence of 2,4‐D in the culture medium promoted callus induction and proliferation, together with abscisic acid (ABA) accumulation, in the hypocotyl segments during the first weeks of culture. When the callus segments generated in the hypocotyl sections cultured in the presence of 2,4‐D were cultivated further, the development of two callus types was observed, one composed of preglobular and globular embryos and the other translucent, watery and lacking any sign of organisation. The embryos of the first type germinated when callus segments were transferred to regeneration conditions, while no change was observed when the second type was induced to regenerate. Higher levels of free IAA and ABA were obtained in the embryogenic calli when compared to the non‐embryogenic, while no differences were observed among callus types in the other hormones evaluated. The possible role of the different plant hormones during induction of somatic embryogenesis is discussed.es_ES
dc.description.sponsorshipGerman Academic Exchange Service//DAAD/Alemaniaes_ES
dc.description.sponsorshipUniversidad de Costa Rica//UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourcePhysiologia Plantarum; vol 111, pp. 389-395es_ES
dc.subjectAgronomíaes_ES
dc.titleEndogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrotes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2019-06-09T17:47:24Z
dc.identifier.doi10.1034/j.1399-3054.2001.1110317.x
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS)es_ES


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