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dc.creatorEl Ekiaby, Magdy
dc.creatorVargas Arroyo, Mariángela
dc.creatorSayed, Makram
dc.creatorGorgy, George
dc.creatorGoubran, Hadi
dc.creatorRadosevic, Mirjana
dc.creatorBurnouf, Thierry
dc.date.accessioned2019-04-08T14:54:58Z
dc.date.available2019-04-08T14:54:58Z
dc.date.issued2015-02-26
dc.identifier.citationhttps://journals.plos.org/plosntds/article/authors?id=10.1371/journal.pntd.0003501es_ES
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/10669/76876
dc.description.abstractPlasma-derived immunoglobulin G (IgG) is on WHO’s Essential Medicines List, yet developing countries face severe shortages of this critical treatment. Infusion of IgG prepared from locally-collected plasma provides an advantageous mix of antibodies to viral and bacterial pathogens found in the living environment, and this can reduce recurrent infections in immune-deficient patients. We developed a simple manufacturing process using disposable equipment (blood bags, hemodialyzer, and filters) to isolate immunoglobulins from minipools of 20 plasma donations. This process yields a ca. 90% pure virally-inactivated immunoglobulin fraction at 50–60% recovery. Anti-hepatitis B and anti-rubella immunoglobulins were enriched fourfold to sixfold. The product was free of in-vitro thrombogenic and proteolytic activity, confirming its expected clinical safety profile. Virus validations showed caprylic acid treatment robustly inactivated or removed infectivity of lipid-enveloped viruses, including human immunodeficiency virus (HIV) and hepatitis C virus model. This simple and cost-effective process is implemented in Egypt to prepare experimental batches for clinical evaluation. It can enhance immunoglobulin supplies to treat immunodeficient patients through passive transmission of antibodies directed against local pathogens. The method requires minimal training and reasonable infrastructure, and is a practical means to prepare convalescent hyperimmune IgG during infectious outbreaks such as the current Ebola episode.es_ES
dc.language.isoen_USes_ES
dc.sourcePLOS Neglected Tropical Diseases, vol. 9(2), e0003501es_ES
dc.subjectCaprylic Acides_ES
dc.subjectDeveloping countrieses_ES
dc.subjectImmunoglobulines_ES
dc.subject616.079 8 Células B (Linfocitos B)es_ES
dc.titleMinipool Caprylic Acid Fractionation of Plasma Using Disposable Equipment: A Practical Method to Enhance Immunoglobulin Supply in Developing Countrieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1371/journal.pntd.0003501
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES


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