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dc.creatorAltamirano Silva, Pamela
dc.creatorMeza Torres, Jazmín Andrea
dc.creatorCastillo Zeledón, Amanda
dc.creatorRuíz Villalobos, Nazareth
dc.creatorZúñiga Pereira, Ana Mariel
dc.creatorChacón Díaz, Carlos
dc.creatorMoreno Robles, Edgardo
dc.creatorGuzmán Verri, Caterina
dc.creatorChaves Olarte, Esteban
dc.date.accessioned2019-02-01T17:08:13Z
dc.date.available2019-02-01T17:08:13Z
dc.date.issued2018-03-22
dc.identifier.citationhttps://iai.asm.org/content/86/4/e00713-17es_ES
dc.identifier.issn1098-5522
dc.identifier.urihttp://hdl.handle.net/10669/76533
dc.description.abstractBrucella abortus is a facultative extracellular-intracellular pathogen belonging to a group of Alphaproteobacteria that establishes close interactions with animal cells. This bacterium enters host cells in a membrane-bound compartment, avoiding the lysosomal route and reaching the endoplasmic reticulum through the action of the type IV secretion system, VirB. In this work, we demonstrate that the BvrR/BvrS two-component system senses the intracellular environment to mount the transcriptional response required for intracellular life adaptation. By combining a method to purify intracellularly extracted bacteria with a strategy that allows direct determination of BvrR phosphorylation, we showed that upon entrance to host cells, the regulatory protein BvrR was activated (BvrR-P) by phosphorylation at aspartate 58. This activation takes place in response to intracellular cues found in early compartments, such as low pH and nutrient deprivation. Furthermore, BvrR activation was followed by an increase in the expression of VjbR and VirB. The in vitro activation of this BvrR-P/VjbR/VirB virulence circuit rescued B. abortus from the inhibition of intracellular replication induced by bafilomycin treatment of cells, demonstrating the relevance of this mechanism for intracellular bacterial survival and replication. All together, our results indicate that B. abortus senses the transition from the extracellular to the intracellular milieu through BvrR/BvrS, allowing the bacterium to transit safely to its replicative niche. These results serve as a working model for understanding the role of this family of two-component systems in the adaptation to intracellular life of Alphaproteobacteria.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B3-761]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourceInfection and Immunity, vol. 86(4), art. e00713-17es_ES
dc.subjectTwo component systemes_ES
dc.subjectType IV secretion systemes_ES
dc.subjectBrucellosises_ES
dc.subjectProteobacteriaes_ES
dc.titleSenses the Intracellular Environment through the BvrR/BvrS Two-Component System, Which Allows To Adapt to Its Replicative Nichees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2019-01-16T17:24:05Z
dc.identifier.doi10.1128/IAI.00713-17
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.identifier.codproyecto803-B3-761


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